南方红豆杉不同部位多糖含量提取分析研究  

Study on the content of polysaccharides in different parts of taxus chinensis var extracts

作　　者：张春椿[1] 李影影[2] 黄孝闻[3] 冯凯波[1] 马多萍 孙瑾[1] 沈津[1] 熊耀康[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江中医药大学附属第二医院,杭州310005 [3]浙江省中医药研究院,杭州310007

出　　处：《中华中医药杂志》2014年第8期2634-2639,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金(No.LQ12H28003);浙江省科技厅项目(No.2012C23109)~~

摘　　要：目的:探究南方红豆杉枝、叶中红豆杉多糖提取最佳工艺条件,分析比较其枝、叶中红豆杉多糖含量。方法:选取新鲜南方红豆杉枝、叶,利用响应面法(RSM)对其枝、叶中红豆杉多糖提取工艺进行优化。在单因素实验基础上选取实验因素与水平,根据中心组合实验设计原理采用3因素3水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以红豆杉多糖提取率为响应面值作响应面和等高线。结果:在分析各个因素的显著性和交互作用后,得出南方红豆杉枝中红豆杉多糖超声最佳提取工艺条件为:水料比24∶1,超声提取功率90W,超声提取时间25min;南方红豆杉叶中红豆杉多糖超声最佳提取工艺条件为:水料比35∶1,超声提取功率70W,超声提取时间30min。南方红豆杉枝中红豆杉多糖一次提取率可达11.6405mg/g,叶中红豆杉多糖一次提取率可达45.8344mg/g。结论:南方红豆杉叶中红豆杉多糖含量比枝中高。Objective： To explore the optimal technological condition on extracting the polysaccharide of taxus mairei from the branch and leaves of taxus chinensis vat, and compare the content of polysaccharides in the branch and leaves of taxus chinensis vat. Methods： The extraction process for taxus mairei polysaccharide was optimized with response surface method （RSM）. The experimental factors and lever were selected on the basis of signal factor. According to the central composite design principle, RSM of 3 factors and 3 levers was used to analyze the influencing factors of technological conditions. The extraction efficiency of axus mairei polysaccharide was used as response and contour line. Results： After analyzing the significance and interactive effects of each factor, a conclusion was drawn： The optimal polysaccharide extraction process in the branch of taxus chinensis var was that liquid to solid ratio of 24：1, ultrasonic extraction power 90W, ultrasonic extraction time 25 minutes; The optimal polysaccharide extraction process in the leaves of taxus chinensis var was that liquid to solid ratio of 35 ： 1, ultrasonic extraction power 70W, ultrasonic extraction time 30 minutes. The once extraction rate of taxus mairei polysaccharide in the taxus cbinensis var branch could be up to 11.6405mg/g, while in the taxus chinensis var leaves 45.8344mg/g. Conclusion： The taxus mairei polysaccharide in the taxus chinensis var leaves is higher than that in the taxus chinensis vat branch.

关 键 词：南方红豆杉枝 南方红豆杉叶 多糖 响应面分析 

分 类 号：R284[医药卫生—中药学]