盆腔器官脱垂不同气虚分级MMP-9、TIMP-1mRNA表达差异性的研究  

Study on the expression of MMP-9 and TIMP-1 mRNA in different qi-deficiency grades of pelvic organ prolapse

作　　者：王小红[1,2] 李奕祺[1] 张凌源 郭晓黎[2] 官涵[2] 叶燕红[2] 李灿东[1] 

机构地区：[1]福建中医药大学,福州350108 [2]福建中医药大学附属人民医院,福州350004

出　　处：《中华中医药杂志》2014年第8期2676-2679,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年基金项目(No.81102713);福建省卫生厅重点项目(No.wzzz0911);陈可冀基金(No.CKJ2010006)~~

摘　　要：目的:分析盆腔器官脱垂(POP)不同气虚分级基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑剂-1(TIMP-1)mRNA表达的差异性。方法:使用实时荧光定量PCR(Real time qPCR)方法检测不同患者MMP-9、TIMP-1 mRNA的表达,分析不同气虚积分分级的POP MMP-9、TIMP-1 mRNA表达的差异性。结果:不同气虚分级的POP患者相关因子MMP-9、TIMP-1 mRNA△Ct值表达差异性有统计学意义(P<0.01)。MMP-9 mRNA的相对表达量:气虚3级为气虚2级的1.4512倍。TIMP-1 mRNA的相对表达量:气虚3级为气虚2级的0.5326倍。结论:不同气虚积分分级的POP患者其MMP-9、TIMP-1 mRNA△Ct值表达的差异性比较有统计学意义,其相对表达量存在一定的倍数关系,可作为评估POP患者气虚程度的参考。Objective： To analyze the expression differences of MMP-9 and TIMP-1 mRNA in different qi-deficiency grades of pelvic organ prolapse （POP）. Methods： The expression of MMP-9 and TIMP-1 mRNA were detected in different patients by using real-time fluorescence quantitative PCR （real-time q-PCR）, and to analyze the expression differences of MMP-9, TIMP-1 mRNA in different qi-deficiency grades. Results： The expression differences of MMP-9 and TIMP-1 mRNA ACt value in different qi-deficiency grades of POP patients had statistical significance （P〈0.01）. Relative expression quantity of MMP-9 mRNA： Qi-deficiency level 3 was 1.4512 times of level 2. Relative expression quantity of TIMP-1 mRNA： Qi-deficiency level 3 was 0.5326 times of level 2. Conclusion： The expression differences of MMP-9 and TIMP-1 mRNA ACt value in different qi- deficiency grades of POP patients had statistical significance. The relative expression quantity had a certain multiple relationship, which could be used as a reference mark for evaluating the degrees of POP patients＇ qi deficiency.

关 键 词：盆腔器官脱垂 证素 实时荧光定量PCR MMP-9 TIMP-1 

分 类 号：R271.9[医药卫生—中西医结合]