TGF-β1对人腹膜间皮细胞分泌细胞外基质和bFGF的影响  

THE EFFECT OF TGF-BETAL ON EXTRACELLULAR MATRIX AND bFGF OF HUMAN PERITONEAL MESOTHELIAL CELL

作　　者：刘映红[1] 袁芳[1] 肖平[1] 李一宁[1] 段绍斌[1] 彭佑铭[1] 李军[1] 夏家辉[1] 刘伏友[2] 

机构地区：[1]中南大学湘雅二院肾内科,长沙410011 [2]中南大学湘雅二医院肾科中南大学肾病研究所,长沙410011

出　　处：《中国现代医学杂志》2002年第14期10-13,共4页China Journal of Modern Medicine

基　　金：教育部高等学校重点实验室访问学者基金 (教技司 2 0 0 0 170 );湖南省自然基金项目 (0 1JJY 2 0 71);湖南省卫生厅基金项目 (2 0 0 1-Y -3 9)

摘　　要：目的 :研究TGF - β1对人腹膜间皮细胞分泌细胞外基质和bFGF的影响。方法 :人腹膜间皮细胞(HPMC)作体外培养 ,在第三代 ,将细胞转板至细胞融合后 ,将其分为对照组 (F12 )和TGF - β1刺激组两组(F12 +TGF - β15ng/ml) ,分别在 2 4 ,4 8h采用ELISA法检测上清液中纤维连接蛋白 (FN)和纤溶酶原激活物抑制剂 (PAI- 1)的含量 ;采用半定量逆转录多原酶链反应 (RT -PCR)检测HPMC细胞内FN ,PAI - 1和碱性成纤维细胞生长因子 (bFGF)mRNA的表达。结果 :HPMC表达FN ,PAI - 1和bFGF ;HPMC在 5ng/mlTGF - β1刺激下分泌FN及PAI - 1明显的增加 ,与对照组比较差异有显著性意义 (P <0 .0 5 ) ;HPMC经TGF- β1作用 12h后 ,FNmRNA ,PAI- 1mRNA和bFGFmRNA半定量结果与对照组相比 ,其表达量分别上调18.5 4 % ,8.6 %和 6 6 .9%。结论 :TGF - β1可促进HPMC合成与分泌细胞外基质和bFGF。Objective:To study the effect of TGF-β1 on extracellular and bFGF secretion of human peritoneal mesothelial cell.Methods:Isolated human peritoneal mesothelial cells (HPMC) was cultured Cultured HPMC were treated with F12 with outserum (control group), F12 with TGF-betal(TGF-betal group),Fibronectin(FN) and Plasminogen activator inhibitor-1(PAI-1) were detected by ELISA method. The expression of FN, PAI-1 and bFGF mRNA were detected by RT-PCR method.Results:HPMC expressed FN, PAI-1 and bFGF. Compared with control, FN and PAI-1 were significant increased stimulated by 5ng/ml TGF-betal at 24 hours and 48 hours( P <0.05); FN,PAI-and bFGF mRNA expression were higher at 12 hours afferstimulation by TGF-betal compared with control group,they increased 18.54%,8.6%,66.9% respectively.Conclusion:TGF-betal can induce extracellular matrix and bFGF synthesis and secretion of HPMC.

关 键 词：TGF-Β1 间皮细胞 细胞外基质 BFGF 碱性成纤维细胞生长因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]