HPLC法测定半枝莲中熊果酸的含量  

Determination of Ursolic Acid in Scutellaria barbata D. Don through High Performance Liquid Chromatography

作　　者：殷文杰[1] 刘艳群[1] 黄祝刚 于亮[1] 吕青涛[1] 蒋海强[1] 巩丽丽[1] 

机构地区：[1]山东中医药大学

出　　处：《山东中医杂志》2014年第7期582-584,共3页Shandong Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81173614)

摘　　要：目的：用HPLC法测定半枝莲药材中熊果酸的含量。方法：采用AgilentSB—C18柱（5μm，4．6ram×250mm），以甲醇-2％乙酸（88：12）等度洗脱，流速1．0mL·min^-1，检测波长210nm，柱温30℃。结果：熊果酸浓度在O．0245-0．4918mg·mL^-1（r=0．9996）范围内线性关系良好，平均加样回收率（n：3）为98．7％（RSD=1．90％），供试品溶液在24h内稳定。结论：该方法精密度良好，准确度高，适合于半枝莲药材中熊果酸含量的测定。．Objective：To determine ursolie acid in Scutellaria barbara D.Don using high peflonnance liquid chromatography （HPLC）. Methods ：Agilent Eclipse SB-C18 column （5μm,4.6 mm×250mm） was adopted with a mobile phase of methanol-2% Acetic Acid（88：12） at the flow rate was 1.0 mL/min,the detection wavelength was set at 210 nm and the column temperature was 30℃. Results：The content of ursolic acid was linear in the ranges of 0.0245-0.4918 mg/mL（r=0.9996） for ursolic acid. The average recoveriestn=3） of ursolic acid were 98.7%（RSD = 1.90%）. The sample solution was steady within 24 h. Conclusi4n：This method is precise and accurate for content determination of ursolic acid in Scutellaria barbara D.Don.

关 键 词：半枝莲 熊果酸 HPLC 含量测定 

分 类 号：R284.2[医药卫生—中药学]