小檗碱脂质体对代谢综合征患者大网膜脂肪组织GSK-3β磷酸化的调节作用  

Effect of Berberine Hydrochloride Liposome on Expression of GSK-3β Phosphorylation in Omental Adipose Tissue of Metabolic Syndrome Patients

作　　者：许旌[1] 张洪[1] 苏爱梅[1] 包三裕[1] 

机构地区：[1]南京大学医学院附属鼓楼医院老年科,江苏南京210008

出　　处：《山东中医药大学学报》2014年第4期389-391,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：南京市卫生局医学科技发展重点项目(编号:ZKX09031)

摘　　要：目的:研究小檗碱脂质体对代谢综合征(MS)患者大网膜脂肪组织糖原合成酶激酶-3β(GSK-3β)及其磷酸化的调节作用。方法:采用不同浓度的小檗碱脂质体干预大网膜脂肪组织,通过葡萄糖氧化酶-过氧化物酶法测定培养上清液中葡萄糖浓度,Realtime PCR检测GSK-3β基因的表达,Western blot测定pGSK-3β、GSK-3β蛋白的表达。结果:与对照组比较,小檗碱原药及脂质体可降低上清液中葡萄糖浓度,显著下调GSK-3β基因的表达,使pGSK-3/GSK-3β蛋白比值显著升高,皆以1×10-6mol/L脂质体组调节作用最为明显。结论:小檗碱脂质体可显著改善脂肪组织的葡萄糖代谢,其机制可能与增加GSK-3β的磷酸化、使活化GSK-3β减少有关。Objective：To investigate the expression of glycogen synthase kinase 3 beta（GSK-3β） phosphory lation of berberine hydrochloride liposome in omental adipose tissue of metabolic syndrome（MS）. Methods：Omental adipose tissues were cultivated and interfered with berberine hydrochloride liposome. The glucose concent rations were measured by GOD-POD method. GSK-3βmRNA in adipose tissue was detected by realtime polymerase chain reaction（realtime-PCR）. Phosphorylated form of GSK-3β was confirmed by Western blot. Results：The glucose concentrations in berberine and hydrochloride liposome were found to be lower than the control group. Berberine hydrochloride liposome treatment decreased GSK-3β expression dose-dependently,but increased pGSK-3β/GSK-3β production sharply. Conclusion：Berberine hydrochloride liposome can improve glucose metabolism of adipose tissues. The mechanism is possibly associated with the increase of GSK-3β phosphorylation.

关 键 词：代谢综合征 脂肪组织 小檗碱脂质体 糖原合成酶激酶-3Β 糖原合成酶激酶-3β磷酸化 

分 类 号：R285.5[医药卫生—中药学]