烟酰胺磷酸核糖转移酶在2型糖尿病大鼠糖代谢相关组织中的表达  

Expressions of Nampt in glucose metabolism related tissues of rats with tyge 2 diabetes mellitus

作　　者：冯乔[1] 王曼伊 王平[2] 胡婷婷[2] 乔伟[2] 梁瑜祯[3] 冯乐平[2] 

机构地区：[1]桂林医学院附属医院优生遗传科,广西桂林541004 [2]桂林医学院生物技术学院生物技术教研室,广西桂林541004 [3]广西医科大学第一附属医院糖尿病代谢中心,广西南宁530001

出　　处：《吉林大学学报（医学版）》2014年第4期739-742,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30860116;31060161);广西壮族自治区科技厅自然科学回国基金资助课题(2010GXNSFC013016);广西壮族自治区教育厅大学生创新创业训练计划项目资助课题(桂教高教[2013]14号)

摘　　要：目的:研究2型糖尿病大鼠机体主要能量代谢器官(肝脏、胰腺、肌肉和肾脏)中烟酰胺磷酸核糖转移酶(Nampt)的表达,探讨Nampt表达和分布与糖尿病发病的关系。方法:腹腔注射链脲佐菌素(STZ)建立SD大鼠糖尿病模型,将大鼠随机分为糖尿病组和对照组。检测大鼠空腹血糖(FBG)和胰岛素分泌水平;采用免疫组织化学Envision染色法检测大鼠肝脏、胰腺、骨骼肌和肾脏组织中Nampt蛋白表达和分布。结果:糖尿病组大鼠FBG水平明显高于对照组(P<0.01),空腹胰岛素水平明显低于对照组(P<0.01)。糖尿病组大鼠肝脏组织Nampt表达明显增高,Nampt蛋白充满全部细胞质;骨骼肌Nampt蛋白表达使整个细胞呈浓染;肾脏细胞Nampt表达主要分布在肾小管上皮细胞内,表达蛋白主要分布于胞浆;与对照组比较,糖尿病组大鼠肝脏、骨骼肌和肾脏组织Nampt阳性表达率明显增高(P<0.05或P<0.01);糖尿病组大鼠胰腺组织几乎未见Nampt表达,Nampt阳性表达率明显低于对照组(P<0.01)。结论:Nampt在糖尿病状态下大鼠主要能量代谢器官中的表达发生明显改变,且呈现出规律性变化,提示Nampt可能成为糖尿病发病过程中的一种特异的指示性标志物。Objective To study the expressions of nicotinamide phosphoribosyl transferase（Nampt）in main energy metabolism organs（liver,pancreas,skeletal muscle,and kidney）of the rats with type 2 diabetes mellitus（T2DM）,and to explore the correlation between the expression and distribution of Nampt and the occurrence of diabetes.Methods The SD rat diabetes model was established by injecting with streptozotcin（STZ）.The SD rats were randomly divided into diabetes group and control group.Immunohistochemical Envision staining assay was used to detect the distribution and protein expressions of Nampt in liver,pancreas,muscle,and kidney tissues of the rats,at the same time the blood glucose and serum insulin levels were also be detected.Results The blood glucose level of the rats in diabetes group was significantly higher than that in control group（P〈0.01）,and the fasting insulin level was lower than that in control group（P〈0.01）.The Nampt expression in the liver tissue of the rats in diabets group was significantly increased,which distributed near the hepatic sinus in diabetes rats,and the Nampt expression was also increased in skeletal muscle in which the whole cell was thick dying;the Nampt expression mainly distributed in the renal tubular epithelial cells.Compared with control group,the positive expression rates of Nampt in liver,skeletal muscle,and kidney tissues of the rats in diabets group were significantly increased（P〈0.05or P〈0.01）.There was nearly no Nampt expression in pancreas tissue of the rats in diabetes group and the Nampt expression level was significantly lower than that in control group（P〈0.01）.Conclusion The Nampt expressions are much different in main energy metabolic organs of the rats with diabetes.It is suggested that Nampt may be used as a specific indicative marker in the process of diabetes.

关 键 词：烟酰胺磷酸核糖转移酶 2型糖尿病 链脲佐菌素 免疫组织化学 

分 类 号：R587.1[医药卫生—内分泌]