新型光动力学疗法联合核仁素沉默对宫颈癌SiHa细胞的光动力学效应  

Photodynamic effect of new photodynamic therapy combined with nucleolin silenceon cervical cancer cell line SiHa

作　　者：刘洪丽[1] 吕昌帅[1] 丁佰娟[1] 王颉[1] 李姗[1] 张友忠[1] 

机构地区：[1]山东大学齐鲁医院妇产科,山东济南250012

出　　处：《吉林大学学报（医学版）》2014年第4期748-752,I0002,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81072122)

摘　　要：目的:研究新型光敏剂阳离子卟啉(TMPyP4)介导的光动力学疗法(PDT)联合人核仁素基因沉默对人宫颈癌SiHa细胞的体外抑瘤效应,探讨有效的宫颈癌联合治疗方案。方法:SiHa细胞分为空白对照组、RNAi组、PDT组和联合治疗组(PDT-RNAi组)。采用细胞增殖及细胞毒性检测试剂盒(CCK8)法检测各组细胞增殖活性;采用流式细胞术定量检测各组细胞凋亡率;采用Tranwell实验检测各组细胞侵袭能力。结果:与空白对照组比较,RNAi组、PDT组和PDT-RNAi组细胞的生长抑制率明显升高(P<0.01);PDT-RNAi组细胞生长抑制率明显高于RNAi组和PDT组(P<0.05),Q值等于1.27。与空白对照组比较,各实验组细胞凋亡率增加(P<0.05),PDT-RNAi组晚期细胞凋亡率明显增加,PDT-RNAi组细胞凋亡率与RNAi组和PDT组比较差异也有统计学意义(P<0.05)。与空白对照组比较,各实验组细胞侵袭性均减弱(P<0.05);PDT-RNAi组细胞侵袭性减弱最为显著,且与RNAi组和PDT组比较差异均有统计学意义(P<0.05)。结论:新型PDT对SiHa细胞具有良好的体外光动力效应,可抑制宫颈癌SiHa细胞的增殖和侵袭,促进其凋亡。核仁素基因沉默对SiHa细胞的PDT具有协同增效作用,PDT联合核仁素沉默可能成为治疗肿瘤的新方案。Objective To study the antitumor effect of new photodynamic therapy（PDT）applying TMPyP4combined with nucleolin silence on cervical cancer SiHa cells in vitro,and to explore an available combination treatment project for cervical cancer.Methods The SiHa Cells were divided into blank control group,RNAi group,PDT group and PDT-RNAi group.The proliferation activities of SiHa cells were assessed by Cell Counting Kit-8（CCK8）assay.The apoptotic rates were measured by flow cytometry（FCM）with AnnexinⅤ/PI staining.The invasiveness abilities were assessed by Transwell assay.Results Compared with blank control group,the inhibitory rates of growth of SiHa cells in RNAi group,PDT group and PDT-RNAi group were increased significantly（P〈0.01）;the inhibitory rates of growth of SiHa cells in PDT-RNAi group were higher than those in PDT group and RNAi group（P〈0.05）.The Q value was 1.27.Compared with blank control group,the apoptotic rates of SiHa cells in experiment groups were increased（P〈0.05）,and the late apoptotic rate in PDT-RNAi group was also increased;there were significant differences of the apoptotic rates between PDT-RNAi group and RNAi group,PDT group（P〈0.05）.Compared with blank control group,the cell invasiveness abilities of SiHa cells in experiment groups were decreased;there were significant differences of the invasiveness abilities of SiHa cells between PDT group and RNAi group（P〈0.05）.Conclusion New PDT shows a strong photodynamic effect on the SiHa cells,which can inhibit the proliferation and invasiveness and induce the apoptosis of SiHa cells in vitro;nucleolin silence shows a good synergy effect to PDT.

关 键 词：宫颈肿瘤 SIHA细胞 新型光动力疗法 核仁素沉默 抗肿瘤效应 

分 类 号：R737.33[医药卫生—肿瘤]