Characterization of expressed genes in the establishment of arbuscular mycorrhiza between Amorpha fruticosa and Glomus mosseae  

作　　者：Fuqiang Song Jize Li Xingxing Zhang 

机构地区：[1]Bio-ecological Restoration, School of Life Science, Heilongjiang University

出　　处：《Journal of Forestry Research》2014年第3期541-548,共8页林业研究（英文版）

基　　金：supported by National Natural Science Foundation of China(31070576 and 31270535);Natural Science Foundation of Heilongjiang Province of China(No.ZD201206);Excellent Youth Foundation of Heilongjiang Province of China(No.JC201306);High-level Talents Support Program of Heilongjiang University(Ecological Restoration Team)

摘　　要：Arbuscular mycorrhiza （AM） formed between plant roots and fungi is one of the most widespread symbiotic associations in nature. To understand the molecular mechanisms of AM formation, we profiled 30 symbiosis-related genes expressed in Amorpha fruticosa roots colonized by Glomus mosseae and in non-mycorrhizal roots at different stages using differential-display RT-PCR （DDRT-PCR）. The expressed genes were confirmed by reverse Northern blotting. Eleven fragments were sequenced and putatively identified by homologous alignment. Of the eleven AM-related genes, five were obtained at the early-stage of plant-fungus interaction and six at the later stage. Three expressed se-quence tag （ESTs） sequences were found to originate from the fungi and eight from the host plant by use of PCR evaluation of gDNA of both plant and fungi. The target genes included an ATP-binding cassette sub-family transporter gene, a transposon-insertion display band, and a photosynthesis-related gene. The results provided information on the molecular mechanisms underlying the development of mycorrhizal sym-biosis between woody plants and AM fungi.Arbuscular mycorrhiza （AM） formed between plant roots and fungi is one of the most widespread symbiotic associations in nature. To understand the molecular mechanisms of AM formation, we profiled 30 symbiosis-related genes expressed in Amorpha fruticosa roots colonized by Glomus mosseae and in non-mycorrhizal roots at different stages using differential-display RT-PCR （DDRT-PCR）. The expressed genes were confirmed by reverse Northern blotting. Eleven fragments were sequenced and putatively identified by homologous alignment. Of the eleven AM-related genes, five were obtained at the early-stage of plant-fungus interaction and six at the later stage. Three expressed se-quence tag （ESTs） sequences were found to originate from the fungi and eight from the host plant by use of PCR evaluation of gDNA of both plant and fungi. The target genes included an ATP-binding cassette sub-family transporter gene, a transposon-insertion display band, and a photosynthesis-related gene. The results provided information on the molecular mechanisms underlying the development of mycorrhizal sym-biosis between woody plants and AM fungi.

关 键 词：arbuseular mycorrhiza differential display gene expression Glomus mosseae 

分 类 号：S793.2[农业科学—林木遗传育种]