变应性鼻炎患者外周血中神经营养因子mRNA的表达及与Th1/Th2免疫失衡的关系  

Neurotrophins up-express in peripheral blood of allergic rhinitis patients and related to Th2 hypothesis

作　　者：王豪[1,2] 陈仁辉[1] 钟燕青[1] 耿义群[3] 许慈燕[3] 康炜骠[1] 林钰龙[4] 

机构地区：[1]汕头大学医学院第二附属医院耳鼻咽喉-头颈外科,广东汕头515021 [2]惠州市第三人民医院,广东惠州516000 [3]汕头大学香港中文大学联合汕头国际眼科中心中心实验室 [4]汕头大学医学院中心实验室

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第14期1024-1027,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省科技计划项目(No:2010B080701053)

摘　　要：目的:测定变应性鼻炎患者外周血中神经生长因子(NGF)、脑源性神经营养因子(BDNF)、神经营养蛋白-3(NT-3)mRNA的表达水平,观察其表达与Th1/Th2免疫失衡的关系。方法:采用成组对照试验,使用实时定量RT-PCR检测外周血中NGF、BDNF、NT-3mRNA的表达水平,2-ΔΔCt法计算实验组基因表达的变化倍数。ELISA检测Th2类细胞因子和Th1类细胞因子的含量。结果:相较于正常成人,变应性鼻炎患者外周血中NGF、BDNF、NT-3mRNA的表达量是正常人的2.44、4.46和1.78倍,NT-3mRNA的表达随着鼻炎严重程度加重呈递增趋势。变应性鼻炎患者外周血中Th2类细胞因子IL-4、IL-6、IL-10的浓度依次为2198±472、9407±703、3917±323μg/L,明显高于正常成人(P<0.05);而Th1类细胞因子INF-a的浓度为176±15μg/L,明显低于正常成人(P<0.05)。NGF、NT-3的扩增水平与IL-4、IL-6、IL-10浓度呈正相关。结论:相较于正常成人,变应性鼻炎患者外周血中NGF、BDNF、NT-3mRNA的表达量上调,NGF、NT-3可能通过Th1/Th2免疫失衡促使变应性鼻炎发病。ObjectiveTo detect the expression of NGF,BDNF,NT-3 mRNA in the peripheral blood of patientswith allergic rhinitis（AR）. And to analyze the correlation between NGF,BDNF,NT-3 mRNA expression and theepidsode of rhinitis through Th-2 Hypothesis. Method：This study was a group controlled trial. The expression ofNGF, BDNF and NT-3 mRNA were tested by real-time quantitative RT-PCR and the concentrations of IL-4, IL-6, IL-10 and INF-a were tested by ELISA. Result.. The expression of NGF, BDNF and NT-3 mRNA in AR pa-tients were 2.44, 4. 46 and 1.78 times the amount of those in the healthy adults, respectively. The increased ex-pression of NT-3 correlated positively with the scores of visual analog scale of AR. The concentrations of IL-4,IL-6 and IL-10, which were 2198 ± 472 μg/mL, 9407± 703 μg/mL and 3917 ±323 μg/mL respectively, werehigher than those in the healthy adults. The concentration of INF-a was 2198 ± 472 μg/mL and less than thehealthy adults. The increased expressions of NGF,NT-3 were positively related to the increase of IL-4, IL-6 andIL-10. Conclusion：The expressions of NGF, BDNF and NT-3 mRNA in AR patients are higher than those in thehealthy adults. NGF, BDNF and NT-3 may contribute to the pathogenesis of AR. Moreover, NGF and NT-3 mayinduce the episode of rhinitis through Th-2 Hypothesis.

关 键 词：鼻炎 变应性 病理生理学 神经营养因子 脑源性神经营养因子 神经营养蛋白-3 白介素-4 白介素-6 白介素-10 干扰素-A 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]