布地奈德预防应用对变应性鼻炎大鼠模型IL-4和IL-5表达影响的研究  

Prophylactic effect of Budesonide on the expression of IL-4、IL-5 in model of allergic rhinitis rats

作　　者：郭小艳[1] 耿曼英[1] 李仲[1] 张慧[1] 魏秀芬[1] 

机构地区：[1]郑州大学第二附属医院耳鼻咽喉科,郑州450003

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第14期1057-1060,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨布地奈德预防性应用对变应性鼻炎最轻持续炎症反应大鼠模型鼻黏膜IL-4、IL-5表达的影响。方法:80只健康SD大鼠,雌雄不限,随机分为变应性鼻炎组(A组,20只)、实验组(B组,20只)、对照组(C组,20只)、空白对照组(D组)。A组制作变应性鼻炎模型,B、C组制作变应性鼻炎最轻持续炎症反应模型,模型建立随后处死一半A、B、C和D组大鼠,观察鼻黏膜嗜酸粒细胞(EOS)浸润和ICAM-1、IL-4、IL-5的表达情况。剩余B组大鼠用布地奈德(64μg,2次/d,每次每侧鼻孔1喷)治疗2周,剩余A、C、D组大鼠用生理盐水喷鼻,2周后3组大鼠均用1%卵清蛋白滴鼻,1次/d,共1周,激发完成后处死全部大鼠,观察鼻黏膜EOS和IL-4、IL-5表达情况。结果:布地奈德药物治疗后,B组平均EOS计数与A、C组相比,差异有统计学意义(P<0.05);B组与D组相比,差异无统计学意义(P>0.05)。B组IL-4灰度值与A、C组相比,差异有统计学意义(P<0.05);A组与C组相比,差异无统计学意义(P>0.05);鼻黏膜组织中IL-5阳性区的灰度值比较结果亦同上。结论:布地奈德最轻持续炎症反应期应用可显著抑制变应。ObjectiveTo explore the prophylactic effect of Budesonide on the expression of IL-4,IL-5 in nasalmucosa in model of minimal persistent inflammation of allergic rhinitis in rats. Method：Eighty SD rats were ran-domly divided into allergic rhinitis group （A group）, experimental （B group）, control group （C group） and nega-tive control group （D group）. A group was made for model of allergic rhinitis. 13 and C group were made for modelof the lightest persistent inflammatory response. After the models were established, half of rats in the A group, Bgroup, C group and D group were executed, and EOS infiltration and the expression of IL-4,IL-5, ICAM-1 wereobserved in nasal mucosa. The remaining rats of B group were given hudesonide （64 /ag/side/time, twice/day）treatment for 2 weeks. A, C, D group were given nasal spray with normal saline for 2 weeks. After that A, B,and C groups were stimulated with 1% OVA daily for one week, D group were given nasal spray with normal sa-line, All rats were executed after excitation, EOS infiltration and IL-4, IL-5 expression were observed. Result： Af-ter the drug treatment,B group only had a small amount of mucous EOS infiltration and had no significant differ-ence with D group, but in A and C group EOS had heavy infiltration. Gray value of the IL-4 positive areas in Bgroup were significantly different compared with A and C group （P〈0. 05）, A group and C group had no signifi-cant difference （P〈0.05）. Distribution of IL-5 positive signals was similar with that of IL-4. Conclusion：Budes-onide MPI application could significantly inhibit the allergic.

关 键 词：鼻炎 变应性 最轻持续炎症反应 布地奈德 IL-4 IL-5 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]