SPRY2正向调节TNF-α诱导的成纤维细胞瘤L929细胞毒作用  

SPRY2 positively regulates the cytotoxicity of TNF-α on fibroblastoma L929 cells

作　　者：朱静潆[1,2] 杜芝燕[2] 王芳[2] 殷小涛[2] 田仁礼[2] 王启宇[2] 武帅[2] 张巍[2] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]军事医学科学院基础医学研究所转化医学研究室,北京100850

出　　处：《免疫学杂志》2014年第7期569-573,共5页Immunological Journal

摘　　要：目的探索SPRY2分子在肿瘤坏死因子α(TNF-α)诱导的L929细胞毒作用中的调节作用。方法应用RT-PCR方法观察SPRY2基因在不同质量浓度TNF-α刺激作用下表达水平的变化;应用重组腺病毒介导SPRY2基因过表达方法观察其对TNF-α细胞毒活性的调解效应。结果 L929细胞中存在SPRY2基因的天然表达,并且其表达水平随TNF-α刺激质量浓度的升高而升高,过表达SPRY2基因促进TNF-α诱导的L929细胞死亡。结论 SPRY2分子在TNF-α介导的肿瘤细胞杀伤效应中发挥正向调节作用。The aim of this study was to explore the regulatory role of SPRY2 in TNF-α-mediated L929 cell cytotoxicity. RT-PCR was employed to detect the changes of SPRY2 expression level after stimulation with different concentrations of TNF-α. Then the regulatory effect of SPRY2 on TNF-α cytotoxicity against L929 cell was observed through SPRY2 gene overexpression mediated by recombinant adenovirus. The native expression of SPRY2 eDNA was found in L929 cells, and its expression level increased as TNF-α stimulation concentration increased. Furthermore, SPRY2 gene overexpression promoted TNF-ct-induced L929 cell death. In conclusion, SPRY2 molecule plays a positively regulatory role in TNF-α-mediated cytotoxic effect on tumor cells.

关 键 词：SPRY2 TNF-Α L929细胞 细胞毒性 

分 类 号：R392[医药卫生—免疫学]