重组人sCR1蛋白对大鼠肢体缺血再灌注损伤的保护作用  

Effects of preconditioning with recombinant soluble complement receptor type 1 protein on limb ischemia/reperfusion injury in rats

作　　者：李琛琪[1] 王广兰[2] 周明武[3] 王飞云[3] 朱杰[3] 罗彦平[1] 

机构地区：[1]新乡医学院,河南新乡453003 [2]解放军第153中心医院检验中心,郑州450042 [3]解放军第153中心医院全军创伤骨科中心,郑州450042

出　　处：《中国矫形外科杂志》2014年第14期1304-1309,共6页Orthopedic Journal of China

基　　金：河南省重点科技攻关项目(编号:082102310046)

摘　　要：[目的]探讨重组人可溶性补体受体1型(Soluble complement receptor type 1,sCR1)对大鼠肢体缺血再灌注(I/R)损伤的保护作用。[方法]采用无创血管夹夹闭左侧股动脉制作大鼠肢体缺血再灌注(Ischemiareperfusion,I/R)损伤模型,观察0、4、8 h时间点sCR1蛋白干预组(B组)与生理盐水(NS)对照组(A组)I/R损伤肌组织细胞形态学变化、测定肌肉湿干重比(W/D)、髓过氧化物酶(MPO)、丙二醛(MDA)、乳酸脱氢酶(LDH)的表达水平。[结果]sCR1蛋白干预后的B组各时间点腓肠肌组织的W/D均低于NS对照A组,以I/R 4 h组最为显著(P<0.01);B组的MDA、MPO、LDH表达水平明显低于A组,以I/R 4 h组最为显著(P<0.01)。[结论]重组人sCR1蛋白对大鼠模型进行sCR1蛋白早期干预后,可明显减轻大鼠后肢骨骼肌I/R的损伤。[ Objective] To determine the effects of recombinant soluble complement receptor type 1 （sCRI） protein on limb ischemia/reperfusion （I/R） injury in rats. [ Method ] Wistar rats in whom an I/R injury was induced using a noninvasive arterial clip were classified into a group injected with normal saline （ NS group） or sCRI （ sCRI group）. To observe the morphological changes of the skeletal muscle, the wet/dry weight ratio （W/D）, and changes in the expression of myeloperoxidase （MPO）, malondialdehyde （MDA）, and lactate dehydrogenase （LDH） by the muscle specimens from both groups were assessed at 0 hour,4 hours,and 8 hours post - injury. [Result]The W/D of the sCR1 group was lower than that of the NS group at all 3 time intervals ,with the difference being the most significant at 4 hours （P 〈 0.01 ）. The expression of MDA and MPO （ except at 0 hour） ,and the LDH expression in the sCR1 group were lower than that in the NS group;the difference was highly significant at 4 hours （P 〈 0.01 ）. [ Conclusion] Early intervention with sCR1 can reduce the limb ischemia/reperfusion injury significantly in an I/R rat model.

关 键 词：缺血再灌注 损伤 重组 可溶性补体受体1型 

分 类 号：R658[医药卫生—外科学]