两品系小鼠食物过敏模型的比较  

Comparison of the BALB /c and Kunming mouse models of food allergy

作　　者：赵新凤[1] 曾本华[2] 谭毅[3] 魏泓[2] 程茜[1] 

机构地区：[1]重庆医科大学附属儿童医院,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]第三军医大学基础部实验动物学教研室,重庆400038 [3]重庆医科大学实验动物学中心,重庆400016

出　　处：《中国实验动物学报》2014年第3期35-39,I0004,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81370906);“973”计划(2013CB531406)

摘　　要：目的：比较两种不同品系小鼠食物过敏模型的敏感性和肠道菌群变化的差异，旨在为食物过敏模型的建立提供依据。方法分别对30只4～5周龄BALB／c和KM雌鼠用卵清蛋白（ ovalbumin，OVA）致敏建立食物过敏模型，ELISA法检测小鼠血清OVA特异性IgE水平；HE染色观察空肠组织形态；采用DGGE技术检测粪便菌群的变化。结果（1）30只致敏的BALB／c小鼠中有27只血清OVA特异性IgE水平明显升高（P＜0.001），而30只致敏的KM小鼠中有21只，且BALB／c小鼠空肠绒毛炎症细胞浸润、上皮脱落及坏死比KM小鼠明显；（2）食物过敏造模后，BALB／c小鼠肠道菌群的改变明显（P＜0.001），而KM小鼠中仅有均匀度改变显著（P＜0.05）；（3） BALB／c小鼠和KM小鼠对照组肠道菌群的丰富度、Shannon指数及均匀度都有差异。结论 BALB／c小鼠对OVA的敏感性高于KM小鼠，不同品系小鼠肠道菌群结构不同，OVA处理后，BALB／c小鼠菌群的改变比KM小鼠更明显。Objective In order to provide the basis for establishment of food allergy models , we compared the differences of sensitivity and alterations of intestinal flora of food allergy models in two strains of mice .Methods Forty 4-5-week old female BALB/c and Kunmimg mice were divided into control group （ n=10） and food allergy goup （ n=30）, respectively.Ovalbumin （OVA） was injected to the mice to establish food allergy models .Serum OVA-specific IgE of the mice was assayed by ELISA .The jejunum tissue was examined by pathology with HE staining .The changes of fecal flora were detected by denaturing gradient gel electrophoresis （DGGE）.Results （1）Among the sensitized 60 mice, OVA-sIgE levels were significantly increased in 27/30 BALB/c mice and 21/30 KM mice compared with those of control groups（P＆lt;0.001）.Moreover, there were more evident inflammatory cell infiltration , epithelial cell shedding and cytolysis in the jejunal villi of BALB/c mice than those of KM mice.（2） After food allergy modeling, there were significant changes of intestinal flora in the BALB/c mice （P〈0.001）, while only significant change of evenness was found in the KM mice （P〈0.05）.（3）There were changes of abundance , Shannon index and evenness of intestinal flora in the model groups of BALB/c and KM mice.Conclusions BALB/c mice are more sensitive to OVA allergy than KM mice .The composition of intestinal flora is different among different strains of mice .The changes of intestinal flora after OVA challenge in BALB /c mice are more obvious than those in KM mice .

关 键 词：小鼠 食物过敏 模型 卵蛋白 敏感性 肠道菌群 

分 类 号：Q95-33[生物学—动物学] R378[医药卫生—病原生物学]