健脾益肾中药对HIV特异性T细胞免疫功能的影响  

Effect of Chinese Drugs for Pi Strengthening Shen Benefiting on the Immunity Function of HIV Patients′ Specific T Cells

作　　者：李伟华[1] 李传云[2] 闫惠平[1] 张欣[1] 赵丹彤[1] 李秀惠[3] 

机构地区：[1]首都医科大学附属北京佑安医院感染与免疫研究中心,北京100069 [2]首都医科大学附属北京佑安医院肝脏移植中心,北京100069 [3]首都医科大学附属北京佑安医院中西医结合中心,北京100069

出　　处：《中国中西医结合杂志》2014年第7期812-815,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家"十二五"传染病重大专项资助项目(No.2012ZX10001001-008);北京市中医药科技发展基金项目(No.JJ2009-31);北京市中医管理局青年科学研究资助项目(No.QN2011-122011);北京佑安肝病艾滋病基金(No.BJYAH-2011-023)

摘　　要：目的探讨健脾益肾中药对HIV/AIDS患者、HIV感染者特异性T细胞免疫功能的影响。方法从2010年6月—2012年6月在北京佑安医院随访的HIV/AIDS感染者中随机抽出健脾益肾中药联合高效抗逆转录病毒疗法(highly active anti-retroviral therapy,HAART)治疗组患者20例,单独HAART治疗组患者23例。检测CD4+T淋巴细胞绝对计数及病毒载量,同时使用HIV全基因重叠多肽作为刺激抗原,应用酶联免疫斑点实验(enzyme-linked immunospot,ELISPOT)技术测定两组HIV特异性T淋巴细胞免疫反应强度。结果两组CD4+T淋巴细胞绝对计数及病毒载量差异无统计学意义(P>0.05)。与单独HAART治疗组比较,健脾益肾中药联合HAART治疗组患者的HIV特异性T细胞免疫应答强度明显增强,差异有统计学意义(P<0.05)。随着治疗时间的延长(6、12、18、24个月),健脾益肾中药联合HAART组HIV特异性T细胞有增强的趋势,但差异无统计学意义(P>0.05)。结论健脾益肾中药治疗可增强HIV/AIDS患者HIV特异性T细胞免疫功能。Objective To explore the effect of Chinese drugs for Pi strengthening Shen benefiting （CDPSSB） on the immunity function of HIV/AIDS patients＇ specific T cells. Methods Totally 20 patients were randomly recruited from the treated group [treated by CDPSSB combined highly active anti-retroviral therapy （HAART）] and 23 patients were randomly recruited from the control group （treated by HAART alone）. All patients were follow-up infected persons form You＇an Hospital between from June 2010 to June 2012. CD4 ＋T absolute counts and HIV viral load were detected. Meanwhile, HIV whole gene overlapping peptides were used as stimulating antigen. The response intensity of HIV specific T cells was detected in the two groups. Results There was no statistical difference in CD4 ＋T absolute counts or HIV viral load between the two groups （P 〉0.05）. The response intensity of HIV specific T cells was significantly enhanced in the treated group, when compared with the control group （P 〈0.05）. Along with elongation of treatment time （6, 12, 18, and 24 months） in the treated group, the response intensity of HIV specific T cells showed enhancing tendency, but there was no statistical difference among these time points（P 〉0.05）. Conclusion CDPSSB could enhance improve the immunity function of HIV specific T cells ,which might be one of its mechanisms.

关 键 词：人类免疫缺陷病毒 健脾益肾中药治疗 高效抗逆转录病毒疗法 酶联免疫斑点试验 T淋巴细胞免疫应答 

分 类 号：R259[医药卫生—中西医结合]