糖克煎剂对糖尿病大鼠肾保护作用及TGF-β_1/Smad4表达的影响  

Renal Protection of Tangke Decoction on Rats with Diabetes and Its Effect on the Expression of TGF-β_1/Smad4

作　　者：王自润[1] 张慧宇[2] 郭敏芳[3] 高志雄 李敬林[5] 

机构地区：[1]山西大同大学医院中医科,大同037009 [2]山西大同大学医学院中医系,大同037009 [3]山西大同大学医学院基础医学系,大同037009 [4]山西大同市中医院肾内科,大同037009 [5]辽宁中医药大学附属医院干部门诊科,沈阳110032

出　　处：《中国中西医结合杂志》2014年第7期826-832,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173153)

摘　　要：目的观察糖克煎剂对早期糖尿病大鼠TGF-β1/Smad4表达的影响,探讨糖克煎剂防治早期糖尿病肾保护作用的效果及机制。方法 SD大鼠随机分为正常对照组(12只)、模型组(10只)、中药预防组(10只)、中药治疗组(10只)、西药对照组(10只)。中药预防组造模成功后立即给予灌服糖克煎剂(18g/kg);其余各组均在造模成功4周后灌胃给药。模型组和正常对照组予等量生理盐水,西药对照组予灌服贝那普利混悬液(1mg/kg),中药治疗组给予灌服糖克煎剂(18g/kg)。中药预防组共给药12周,中药治疗组及西药对照组给药8周,均每日1次。实验结束检测各组大鼠体重、肾重、肾重指数、空腹血糖和24h尿微量白蛋白排泄率;HE染色、Masson染色及电镜观察各组大鼠肾组织病理形态学变化;采用免疫组化法检测肾组织转化生长因子(TGF-β1)及其内部传导分子Smad4的表达。结果与正常对照组比较,模型组大鼠体重明显降低,肾重、肾重指数、血糖、24h尿蛋白定量、尿蛋白排泄率、肾脏TGF-β1和Smad4的表达均明显增加,差异均有统计学意义(P<0.01);各用药组上述指标与模型组比较均有所改善,差异均有统计学意义(P<0.05,P<0.01);与西药对照组比较,中药预防组肾重、肾重指数、血糖、24h尿蛋白定量及尿蛋白排泄率明显改善,差异有统计学意义(P<0.01)。肾脏病理变化以模型组最为明显,各用药组均有不同程度改善。结论糖克煎剂可明显改善糖尿病症状,并且下调早期糖尿病大鼠肾脏TGF-β1和Smad4的表达。说明糖克煎剂对早期糖尿病肾病有一定的防治作用。Objective To observe the effect of Tangke Decoction （TD） on the expression of TGF- β1/Smad4 of rats with early diabetes and to explore the effect and mechanism of TD against the renal injury induced by diabetes. Methods SD rats were randomly divided into the normal control group （n =12）, the model group （n =10）, the Chinese herbs prevented group （n =10）, the Chinese herbs treated group （n = 10）, and the Western medicine control group （n =10）. TD （18 mg/kg） was given by gastrogavage to rats in the Chinese herbs prevented group immediately after successful modeling for 12 weeks, once daily. At the 4th week of successful modeling, rats in the rest 4 groups were administered by gastrogavage. Equal volume of normal saline was given to rats in the model group and the normal control group. Benazepril suspension （1 mg/kg） was administered by gastrogavage to rats in the Western medicine control group for 8 weeks, once daily. TD （18 mg/kg） was given by gastrogavage to rats in the Chinese herbs treated group for 8 weeks, once daily. The body weight,kidney weight,index of kidney weight, fasting blood sugar, 24 h u- rinary albumin excretion rate were examined after experiment. The pathological changes of the renal tissue were observed by HE staining, Masson staining, and electron microscope. The expression of renal trans- forming growth factor-β1 （TGF-β1） and Smad4 were detected using immunohistochemical assay. Results Compared with the normal control group, the body weight of rats decreased significantly; the kidney weight, index of kidney weight, blood sugar, 24 h urinary protein excretion, the urinary albumin excretion rate ,TGF-β1 and Smad4 expression increased significantly in the model group （all P 〈0.01 ）. Compared with the model group, the aforesaid indices were improved in each treatment group with statistical difference （P 〈0.05, P 〈0.01 ）. Compared with the Western medicine control group, the kidney weight, index of kidney weight, blood sugar, 24 h urinary p

关 键 词：糖尿病肾病 糖克煎剂 洛汀新 转化生长因子-Β1 SMAD4 

分 类 号：R285.5[医药卫生—中药学]