姜黄素对人循环纤维细胞增殖及COLⅠ表达影响的研究  

Effects of curcumin on proliferation and COL I expression of human circulating fibrocytes

作　　者：富徐燕[1] 赵丕文[1] 李亚东[1] 唐雨晴[1] 牛建昭[1] 王鸿捷[2] 李彧[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京市红十字血液中心,北京100088

出　　处：《中国药理学通报》2014年第7期942-947,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81173642);教育部国家外专局创新引智基地项目(No B07007)

摘　　要：目的从外周血中分离并鉴定人循环纤维细胞,初步探讨姜黄素对人循环纤维细胞的药理作用。方法密度梯度离心法分离纯化细胞,流式细胞术和免疫化学法对细胞进行鉴定;CCK-8法及流式细胞技术分别检测姜黄素对人循环纤维细胞增殖和COLⅠ表达的影响。结果在体外成功分离培养了人循环纤维细胞,细胞呈CD34、CD45和COLⅠ阳性,其中79.7%的细胞为COLⅠ和CD45双阳性,可证明为人循环纤维细胞。姜黄素对循环纤维细胞的增殖起调控作用,不同浓度姜黄素的短时间(24 h)作用可促进人循环纤维细胞的增殖;而作用较长时间(72 h)后不仅能明显抑制细胞的增殖,而且能下调COLⅠ的表达,以高浓度效果最为明显(20μmol·L-1)。结论 20μmol·L-1的姜黄素作用72 h可明显抑制人循环纤维细胞的增殖和COLⅠ的表达。Aim To isolate and characterize the human circulating fibrocytes from human peripheral blood and explore the effects of curcumin on human circulating fi-brocytes.Methods The cells were isolated and puri-fied by density gradient centrifugation,and identified by flow cytometry and immunocytochemistry .Then ,＆amp;nbsp;CCK-8 and flow cytometry were used to study the effect of curcumin on the proliferation as well as COL I ex-pression of human circulating fibrocytes,respectively. Results After being isolated the cells expressed CD34,CD45 and COLⅠ,among which 79.7% were both CD45 and collagen I positive,typical of human circulating fibrocytes.Curcumin could exert regulatory effects on proliferation of human circulating fibrocytes. Exposure of the cells to curcumin for as short as 24 hours promoted their growth,while prolonged treatment＆amp;nbsp;（72 h ） significantly inhibited cell propagation and downregulated the COLⅠ levels,best manifested at a concentration as high as 20 μmol · L-1 .Conclusion The proliferation of cells and COLⅠexpressions can be effectively inhibited by curcumin with the prolonged action period and high concentrations.

关 键 词：人循环纤维细胞 姜黄素 COL I CD34 CD45 鉴定 增殖 表达 

分 类 号：R282.71[医药卫生—中药学] R329.24[医药卫生—中医学]