糖尿病及其并发症患者血浆中左卡尼汀及其酰化物的含量分析研究  

Content of L-carnitine and its acylates in plasma of patients with diabetes and diabetes complications

作　　者：曹玉[1,2] 李萍[2] 徐毅君[2] 王春波[3] 管华诗[1] 

机构地区：[1]中国海洋大学医药学院 [2]青岛大学医学院附属医院,山东青岛266003 [3]青岛大学医学院,山东青岛266071

出　　处：《中国药理学通报》2014年第7期952-955,共4页Chinese Pharmacological Bulletin

基　　金：国家科技支撑计划项目(No 2008BAI54B02);青岛市科技计划基础研究项目[No 11-2-4-2-(3)-jch]

摘　　要：目的探究糖尿病及其并发症患者体内左卡尼汀(Lcarnitine,LC)、乙酰左卡尼汀(acetyl-L-carnitine,ALC)和丙酰左卡尼汀(propionyl-L-carnitine,PLC)的含量变化。方法复制柱前衍生化HPLC同时检测血浆中LC、ALC、PLC的含量的方法,检测并比较健康受试者、糖尿病患者以及糖尿病并发症患者(糖尿病视网膜病变、糖尿病肾病、糖尿病周围神经病变、糖尿病并高血压、糖尿病并冠心病)体内3种物质含量的变化。结果健康受试者、糖尿病患者以及糖尿病视网膜病变、糖尿病肾脏病变、糖尿病周围神经病变、糖尿病并高血压、糖尿病并冠心病患者血浆LC浓度分别为(41.01±8.05)μmol·L-1、(36.72±8.23)μmol·L-1、(33.31±6.26)μmol·L-1、(33.81±5.61)μmol·L-1、(33.57±6.67)μmol·L-1、(33.67±5.36)μmol·L-1、(32.87±6.05)μmol·L-1。糖尿病组的血浆LC浓度明显低于健康对照组(P<0.05),其他并发症组(糖尿病视网膜病变组、糖尿病肾脏病变组、糖尿病周围神经病变组、糖尿病并高血压和糖尿病并冠心病组)的LC浓度又明显低于糖尿病组,但各并发症的血浆LC浓度组间比较无差异(P>0.05),ALC和PLC的检测结果显示各组之间差异并没有显著性(P>0.05)。结论在中国健康受试者、DM患者以及并发症患者中,DM患者的LC血浆浓度低于健康受试者,糖尿病并发症患者LC的血浆浓度低于DM患者。Aim To investigate the changes of content of L-carnitine （LC ）, acetyl-L-carnitine （ALC ） and propionyl-L-carnitine （PLC ）in patients with diabetes and its complications.Methods By replicating meth-od of pre-column derivatization HPLC,the contents of plasma LC,ALC,PLC were detercted in normal sub-jects and in patients of diabetes mellitus,diabetic reti-nopathy, diabetic nephropathy, diabetic peripheral neuropathy,diabetic hypertension,diabetic coronary heart disease.Results The concentration of LC in normal subjects and in patients of diabetes mellitus, diabetic retinopathy, diabetic nephropathy, diabetic peripheral neuropathy,diabetic hypertension,diabetic coronary heart disease were （41.01 ±8.05 ）μmol · L-1 ,（36.72 ±8.23 ）μmol · L-1 ,（33.3 1 ±6.26 ）μmol·L-1 ,（33.81 ±5.61 ）μmol·L-1 ,（33.57 ± 6.67 ）μmol · L-1 , （33.67 ±5.36 ）μmol · L-1 , （32.87 ±6.05 ）μmol · L-1 respectively.The plasma＆amp;nbsp;LC concentration in diabetic group was significantly lower than that of normal control group （P＆lt;0.05 ）. Moreover,the LC concentration in other groups of dia-betic complications was lower than that in diabetic group, and LC concentration showed no significant difference in various groups of diabetic complications. There was no significant difference in the plasma con-centrations of ALC and PLC of diabetes group and the diabetic complication groups too.Conclusion In Chi-nese normal subjects,patients with diabetes and dia-betic complications,the plasma concentrations of LC in diabetic patients are lower than normal,and plasma concentration of LC in diabetic complications are lower than that in patients with diabetes mellitus.

关 键 词：糖尿病 糖尿病并发症 左卡尼汀 乙酰左卡尼汀 丙酰左卡尼汀 高效液相色谱法 

分 类 号：R446.11[医药卫生—诊断学] R587.1[医药卫生—临床医学]