硫化氢对离体大鼠急性缺血心肌心功能的影响  

Effects of hydrogen sulfide on cardiac function induced by acute myocardial ischemia in isolated hearts of rats

作　　者：谢英花[1] 马燕山[2] 张楠[3] 张建新[3] 

机构地区：[1]河北科技大学药学系,河北石家庄050018 [2]石家庄市中医院放射科,河北石家庄050051 [3]河北省医学科学院,河北石家庄050021

出　　处：《中国药理学通报》2014年第7期1000-1006,共7页Chinese Pharmacological Bulletin

基　　金：河北省自然科学基金资助项目(No C2009001458);河北省应用基础研究计划重点基础研究项目(No 13967602D)

摘　　要：目的观察离体大鼠急性缺血心肌内源性硫化氢/胱硫醚-γ-裂解酶(H2S/CSE)体系的动态变化,研究H2S对急性心肌缺血损伤大鼠心脏功能,心肌组织H2S/CSE及心肌梗死体积的影响。方法应用Langendorff心脏灌注系统建立急性心肌缺血损伤模型。分别于相应时间点取材,取材前应用Powerlab/8s多导生理仪记录各组大鼠左室发展压(LVDP)、左室压力变化速率(±dp/dtmax);测定冠脉流量(CF);检测心肌组织中H2S含量及CSE活性;以Evan's蓝和TTC双染法检测心肌梗死体积。结果①与相应假手术组比较,缺血30 min、1、2、3、4 h组LVDP、±dp/dtmax、CF明显降低(P<0.01)。缺血30 min组H2S含量和CSE活性无明显变化,心肌组织无明显梗死灶,缺血1、2、3、4 h组H2S含量和CSE活性均明显降低(P<0.05或P<0.01)。缺血1h组可见明显梗死灶(P<0.01),缺血2、3、4 h组梗死体积明显增大(P<0.01);②与缺血4 h组比较,NaHS低、中、高剂量组LVDP、±dp/dtmax、CF明显升高(P<0.05或P<0.01),H2S含量和CSE活性均升高(P<0.05或P<0.01),NaHS中、高剂量组心肌梗死体积明显减小(P<0.01)。结论H2S与CSE参与了离体大鼠急性心肌缺血损伤。NaHS可增强急性缺血损伤心肌CSE活性,增加H2S含量,使心肌梗死体积缩小,提示H2S可改善急性心肌缺血损伤。Aims To observe the changes of endoge-nous hydrogen sulfide/cystathionine-γ-lyase （H2 S/CSE）system and to study the effects of H2 S on cardiac function,H2 S/CSE and myocardial infarct volumes in acute myocardial ischemic injury in isolated hearts of rats.Methods The myocardial ischemic injury model was established by the ligation of coronary artery.The hemodynamic parameters,such as the left ventricular developed pressure （LVDP）,±dp/dtmax and coronary arterial flow（CF）,were respectively recorded to evalu-ate the cardiac function.The content of H2 S and the activity of CSE in cardiac tissue were detected respec-tively at each time point after ischemia.Infarct vol-umes in isolated rat hearts were determined by dual staining with Evans-blue and TTC.Results （1 ）Com-pared with those of the sham group,LVDP,±dp/dtmax and CF were significantly decreased at 30 min,1,2,3, 4 h after ischemia（P〈0.01）,there were no statistical-ly significant differences in the content of H2 S and the activity of CSE in cardiac tissue at 3 0 min after ische-mia.But during the periods from 1 h to 4h after ische-mia,the content of H2 S and the activity of CSE in car-diac tissue were significantly decreased,the infarct volumes were greatly increased compared with those of the sham control group （P〈0.05 or P〈0.01 ）.（2） Compared with those of the ischemia 4h group,LVDP, ±dp/dtmax and CF were significantly increased in the NaHS low,middle and high dose groups （P〈0.05 or P〈0.01）,the content of H2S and the activity of CSE in cardiac tissue were significantly increased in the NaHS low,middle and high dose groups（P〈0.05 or＆amp;nbsp;P〈0.01 ）,and the infarct volumes were significantly decreased in NaHS middle and high dose groups（P〈0.01 ）.Conclusion H2S and CSE are involved in myocardial ischemic injury in isolated hearts of rats. Administration of NaHS could enhance the activity of CSE,increase the content of H2 S,and reduce infarct volumes.It could be suggested that H2 S has cardiopro-tective

关 键 词：硫化氢 急性心肌缺血 LANGENDORFF 大鼠 心功能 胱 硫醚-γ-裂解酶 

分 类 号：R332[医药卫生—人体生理学] R322.11[医药卫生—基础医学]