芫荽遗传多样性RAPD分析  

RAPD Analysis of Genetic Diversity for Coriander

作　　者：李小梅[1] 张立微[1] 张景涛[1] 

机构地区：[1]哈尔滨市农业科学院蔬菜花卉分院,黑龙江哈尔滨150029

出　　处：《北方园艺》2014年第15期97-100,共4页Northern Horticulture

摘　　要：以96份芫荽为试材,采用RAPD分析方法,研究了芫荽种质资源遗传多样性。结果表明:从300条随机引物中筛出23条扩增性强、清晰度高和稳定性好的引物,扩增出53条带,其中34条为多态性带,多态性比例64.2%。通过聚类分析,生成96份芫荽亲缘关系树状图,在遗传距离0.50处,试材划分为6类。RAPD分析揭示了芫荽的遗传背景并为其分类提供了依据。Taking 96 coriander samples as materials, using the RAPD analysis method, the genetic diversity of germplasm resources of coriander was studied. The results showed that 23 random primers were screened from 300 primers that were amplification of strong, high resolution and good stability. 34 bands were polymorphie among 53 bands identified. The percentage of polymorphic loci was 64. 2%. All the germplasm could be divided into six categories at the genetic distance 0. 50 by cluster analysis. RAPD analysis reflected the complex genetic background and provided the supporting to the identification and classification of coriander.

关 键 词：芫荽 RAPD 遗传多样性 

分 类 号：S573.2[农业科学—作物学]