桔梗胚珠培养中愈伤组织诱导培养基的优化  

Optimization of Media for Inducing Ovule Culture of Platycodon grandiflorum

作　　者：徐芳芳[1] 赵丽莉[1] 荀洋 杨丽[1] 金江山[1] 严一字[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边朝鲜族自治州种子管理站,吉林延吉133001

出　　处：《北方园艺》2014年第15期106-108,共3页Northern Horticulture

摘　　要：以桔梗未受精胚珠为试材,研究比较了不同生长素和细胞分裂素组合对其愈伤组织诱导及鲜重大小的影响,以筛选最佳诱导培养基。结果表明:诱导桔梗未受精胚珠愈伤组织形成的比较理想的培养基组合有2个,一是N6+1.0mg/L 2,4-D+0.1mg/L KT,其诱导率最高,达到了100%,同时愈伤组织平均鲜重较大;二是N6+0.5mg/L NAA+0.1mg/L KT,其诱导率较高为90%,愈伤组织平均鲜重最大。Taking unfertilized ovules of Platycodon grandiflorum as materials, the effect of different combinations of auxin and cytokinin on the callus induction and fresh weight were studied and compared,in order to screen out the best induction medium. The results showed that two optimal media for inducing unfertilized ovules to form calli were selected, the first optimal medium was N6＋1.0 mg/L 2,4-D＋0. 1 mg/L KT,the induction rate was 100% and the average fresh weight of callus was large;the second optimal medium was N6＋0. 5 mg/L NAA＋0. 1 mg/L KT,the induction rate was 90% and the average fresh weight of callus was the largest.

关 键 词：桔梗 胚珠 组织培养 诱导率 愈伤组织鲜重 

分 类 号：Q949.783.2[生物学—植物学]