高效液相色谱-柱后光化学衍生-荧光检测法测定强化食品中的叶酸  

Determination of folic acidin fortified foods by high performance liquid chromatography with post-column photochemical derivatization

作　　者：赵海燕[1] 杨永红[1] 刘泰然[1] 李兵[1] 赵榕[1] 

机构地区：[1]北京市疾病预防控制中心,北京100013

出　　处：《中国食品卫生杂志》2014年第4期347-350,共4页Chinese Journal of Food Hygiene

摘　　要：目的建立柱后光化学衍生一高效液相色谱法（HPLC）测定强化食品中叶酸的分析方法。方法采用SunFireC18（4．6mm×250mm，5μm）色谱柱，以乙腈-50mmol／L磷酸二氢钾溶液（pH=5．0）为流动相梯度洗脱，叶酸在254nm紫外光照射下进行柱后衍生，荧光检测器检测，激发和发射波长分别为344和428nm。结果本方法的线性范围为0．01～2．0μg／ml，r=0．9999；方法的定性检出限和定量检出限分别为0．01和0．03μg／g；加标回收率为90．0％．98．0％，RSD均小于5％。结论本方法简便、快速、准确、重现性好，适用于强化食品中叶酸含量的测定。Objective To establish a method for the determination of folic acid in fortified foods by high performance liquid chromatography （HPLC） with postcolumn photochemical derivatization. Methods Chromatographic separation was carried out using a SunFire C18 column （4. 6 mm× 250 mm, 5 μm）. The mobile phases was acetonitrile and 50 mmol/L potassium dihydrogen phosphate solution （pH = 5.0 ） with an optimized gradient elution. Post-column photochemical derivatization of folic acid was under UV-light irradiation at 254 nm. The fluorescence detection was performed with the excitation at 344 nm and the emission at 428 nm. Results The linear range was 0.01-2.0 μg/ml, r = 0. 999 9. The limit of detection （LOD） was 0.01 μg/g and the limit of quantification （LOQ） was 0.03 μg/g. The average recoveries were 90.0%-98.0% and the RSD of the method was less than 5%. Conclusion The method was simple, rapid, accurate, reproducible, and suitable for the determination of folic acid in fortified foods.

关 键 词：高效液相色谱 柱后光化学衍生 叶酸 强化食品 强化营养素 

分 类 号：O657[理学—分析化学] TS207.3[理学—化学]