切换波长HPLC法同时测定大鼠体内补骨脂3种有效成分  

Simultaneous Determination of 3 Kinds of Active Components in Psoralea corylifolia of Rats by Wavelength Switching HPLC Method

作　　者：张昀[1,2] 刘路[2] 

机构地区：[1]河南大学民生学院,河南开封475004 [2]河南大学药学院,河南开封475004

出　　处：《中国药房》2014年第31期2887-2889,共3页China Pharmacy

基　　金：河南省教育厅研究项目(No.14B350009);河南大学民生学院教育教学改革研究项目(No.MSJG2013-41)

摘　　要：目的:建立同时测定大鼠血清、肝脏、肾脏中补骨脂素、异补骨脂素、补骨脂酚含量的方法。方法:大鼠灌胃补骨脂药材粉末(3.0 g/kg)30 min后取血清、肝脏、肾脏样品,以切换波长高效液相色谱法测定血清、肝脏、肾脏样品中补骨脂素、异补骨脂素、补骨脂酚质量浓度。色谱柱为Agilent ODS-C18(150 mm×4.6 mm,3.5μm),流动相为0.1%甲酸水-乙腈(梯度洗脱),流速为1.0ml/min,柱温为30℃,检测波长为295 nm(氯霉素)、246 nm(补骨脂素、异补骨脂素)、260 nm(补骨脂酚)。结果:在大鼠血清、肝脏匀浆液、肾脏匀浆液中,补骨脂素、异补骨脂素、补骨脂酚质量浓度均在0.1~10.0 mg/L范围内同各指标成分与内标物峰面积之比呈良好线性关系,精密度的RSD<7%,加样回收率的RSD<5%。结论:该方法简便、准确,可用于同时测定给药后血清、肝脏、肾脏中补骨脂素、异补骨脂素和补骨脂酚的含量。OBJECTIVE： To establish a method for the content determination of psoralen, isopsoralen and bakuchiol in serum, liver and kidney of rats. METHODS ： The rats were given Psoralea corylifolia powder 3.0 g/kg intragastrically.The serum, liver and kidney homogenate were collected at 30 min. Wavelength switching HPLC method was adopted to determine the concetrations of psoralen, isopsoralen and bakuchiol.The determination was performed on Agilent ODS-C^8 （150 mm ~ 4.6 ram, 3.5 ~tm） column with mobile phase consisted of 0.1% formic acid-acetonitrile （gradient elution） at the flow rate of 1.0 ml/min. The column temperature was 30 ~C.The detection wavelength was set at 295 nm for chloramphenicol, 246 nm for psoralen and isopsoralen and 260 nm for bakuchiol. RESULTS： All of the three components showed good linear relationship at the range of 0.1-10.0 mg/L, and which can be separated in serum, liver and kidney homogenate. RSDs of precision tests were less than 7% , RSDs of recoveries were less than 5% . CONCLUSIONS： This method is simple and accurate, and can be used for simultaneous determination of psoralen, isopsoralen and bakuchiol in serum, liver and kidney of rats.

关 键 词：切换波长法 高效液相色谱法 补骨脂素 异补骨脂素 补骨脂酚 血药浓度 

分 类 号：R285.5[医药卫生—中药学] R969.3[医药卫生—中医学]