HPLC法测定辽源七厘复方中药控释微丸中龙血素B的含量  

Content Determination of Loureirin B in Liaoyuan Qili Compound TCM Controlled-release Pellets by HPLC

作　　者：刘应杰[1] 杨宗发[1] 何文生 江尚飞[1] 谭小梅[1] 何静[1] 邱妍川[1] 

机构地区：[1]1重庆医药高等专科学校药学院,重庆401331 [2]重庆市大足区人民医院药剂科,重庆402360

出　　处：《中国药房》2014年第31期2929-2930,共2页China Pharmacy

基　　金：重庆市卫生局中医药科研计划立项(No.2011-2-171)

摘　　要：目的：改良测定辽源七厘复方中药控释微丸中龙血素B含量的方法。方法：采用高效液相色谱法。色谱柱为KromasilC18（250mm×4．6mm，5μm），流动相为甲醇．1％冰醋酸溶液（梯度洗脱），流速为1．0ml／min，检测波长为206nm。结果：龙血素B的质量浓度在0．40-20．00μg／ml范围内与峰面积积分值呈良好线性关系（r=0．9995）；精密度、稳定性、重复性试验的RSD〈2％；平均加样回收率为100．50％，RSD=1．90％（n=6）。结论：该改良方法操作简便、分离效果好、灵敏度高、重复性好，可用于辽源七厘复方中药控释微丸的质量控制。OBJECTIVE ： To improve the method for content determination of loureirin B in Liaoyuan qili compound TCM controlled-release pellets. METHODS： HPLC method was adopted. The determination was carded out on Kromasil C18 （250 mm×4.6mm,5μm） column with mobile phase consisted of methanol-1% glacial acetic acid （gradient elution） at a flow rate of 1.0 ml/min. The detection wavelength was set at 206 nm. RESULTS： The linear range of loureirin B was 0.40-20.00 μg/ml（r=0.999 5）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2%. The average recoveries was 100.50% （RSD=1.90%, n=6）. CONCLUSIONS： The improved method is simple, well-separated, sensitive and reproducible, and can be used for the quality control of Liaoyuan qili controlled-release pellets.

关 键 词：辽源七厘复方中药控释微丸 龙血素B 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]