PKC-β抑制剂LY333531对糖尿病大鼠心肌功能及Caveolins蛋白的影响  

Study on the effect of LY333531 on myocardiac function and expression of myocardial protein Caveolins in diabetic rats.

作　　者：雷少青[1] 苏娃婷[1] 徐金金[1] 刘慧敏[1] 夏中元[1] 夏正远[2] 徐波[3] 

机构地区：[1]武汉大学人民医院麻醉科,湖北武汉430060 [2]广东医学院附属医院麻醉科,广东湛江524001 [3]首都医科大学附属北京友谊医院呼吸内科,北京100050

出　　处：《临床和实验医学杂志》2014年第16期1305-1308,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金资助项目(编号:81300674)

摘　　要：目的观察PKC-β抑制剂LY333531(LY)对糖尿病大鼠心肌功能及Caveolins蛋白的影响。方法 24只SD雄性大鼠随机分为正常对照组(C)、糖尿病组(D)及糖尿病LY治疗组。4周后生化检测血浆甘油三酯(TG)、炎症因子TNF-α与IL-6水平,心脏超声评价心脏功能,Western Blot分析心肌组织Cav-1、Cav-3,PKC-β2蛋白表达水平。结果与C组比较,D组大鼠血浆TG、TNF-α与IL-6水平均增高,心脏舒张功能降低,心肌组织p-PKC-β2、Cav-1表达水平增加而Cav-3表达水平降低。与D组比较,LY除了不能降低糖尿病大鼠血浆TNF-α与IL-6水平外,均可明显抑制上述变化。结论 PKC-β抑制剂LY明显改善糖尿病心肌舒张功能,其机制可能与抑制PKC-β2过度活化并恢复Caveolins蛋白表达水平有关。Objective To study the effect of LY333531（LY）on myocardiac function and expression of myocardial protein Caveolins in diabetic rats. Methods A total of 24 male SD rats were randomly divided into control group（C）,diabetic group（D）and diabetic group with LY treatment for 4 weeks. The plasma levels of triglyceride（TG）,inflammatory factor TNF - α and IL -6 were detected by biochemical analysis. The cardiac function was evaluated by echocardiography. The expression of Cav -1,Cav -3 and PKC - β2 were detected by Western blot. Results In comparison with group C,the plasma levels of TG,TNF - α and IL -6 in group D were significantly increased and accompanied with insulted cardiac diastolic function. The protein expression of PKC - β2 and Cav -1 in diabetic rats was up - regulated,but the protein expression of Cav - 3 was down - regulated as compared with that of control rats. The treatment with LY prevented or reversed all these changes without impacting plasma levels of TNF - α and IL -6. Conclusion Selective inhibition of PKC - β with LY can significantly improve diabetic cardiac diastolic function, it may be related to the inhibition of excessive activation of PKC - β2 and restore the expression of Caveolins.

关 键 词：大鼠 糖尿病 PKC-β抑制剂 LY333531 心肌功能 Caveolins 

分 类 号：R587.2[医药卫生—内分泌]