基于猪链球菌2型烯醇化酶建立的筛查并监控细菌侵袭性感染的ELISA方法  

AN ELISA METHOD BASED ON RECOMBINANT ENOLASE SCREENING AND INVASIVE INFECTION MONITORING OF S. SUIS 2

作　　者：孙雯[1] 潘秀珍[2] 

机构地区：[1]扬州职业大学,江苏扬州225000 [2]南京军区军事医学研究所,江苏南京210002

出　　处：《井冈山大学学报（自然科学版）》2014年第4期80-83,92,共5页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(30670105;30600533);国家863计划项目(2006AA0Z455);江苏省科技计划项目(自然科学基金项目)(BK2007013)

摘　　要：目的探索猪链球菌2型(S.suis2)重组烯醇化酶(Enolase)蛋白的免疫学特性,建立调查S.suis2侵袭性感染的标记和方法,并用于人群及猪群中S.suis 2感染的监控和筛选有效的疫苗抗原。方法 PCR法检测enolase在各血清型菌株中的分布情况,免疫印记检测Enolase的免疫反应性,建立筛查S.suis 2感染的酶联免疫吸附试验(ELISA)方法。结果 PCR检测显示enolase在多种血清型中广泛存在,Western-blot表明重组Enolase蛋白具有较好的免疫反应性。基于Enolase建立的ELISA结果显示,感染S.suis 2的猪血清中Enolase抗体效价较高。结论基于Enolase建立的ELISA方法能有效地用于S.suis 2感染的筛查和监控。Objective： To study the immunological characters of recombinant Enolase from S. suis 2, establish a mark and a method for investigating its invasive infection and apply them in monitoring its infection and screening valid vaccine antigen among crowd and swinery. Methods： PCR was adopted to analyze the distribution of enolase gene in all kinds of S. Suis serotype, western-blot experiment was used to detect the immunoreactivity of Enolase, and eno-based ELISA method was established for screening S. suis 2 infection. Results： PCR results showed that enolase gene widely existed in many serotypes of S. suis, western-blot experiment demonstrated recombinant Enolase had stronger immunoreactivity. Evaluation of Eno-based ELISA indicated that serum samples from pigs with S. suis 2 infection have higher levels of antibody titers. Conclusion： ELISA provided an efficient Eno-based method for screening and monitoring the infection ofS. suis 2.

关 键 词：猪链球菌2型(S.suis 2) 烯醇化酶Enolase 酶联免疫吸附试验 ELISA 

分 类 号：S855.99[农业科学—临床兽医学]