巴西橡胶树FCA基因的克隆及功能分析  

Cloning and functional analysis of FCA gene in Hevea brasiliensis

作　　者：鲁旭[1,2] 孙芳[2] 华玉伟[2] 黄华孙[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院橡胶研究所/农业部橡胶树生物学重点开放实验室,海南儋州571737

出　　处：《广东农业科学》2014年第15期116-120,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31200503);国家现代农业产业技术体系建设专项(CARS-34)

摘　　要：FCA基因是植物自主开花途径中的重要调控基因,已被证实是促进植物开花的保守基因。研究通过巴西橡胶树甲基化文库获得橡胶树HbFCA基因一段434 bp的保守cDNA序列,应用RACE技术,成功克隆得到橡胶树的HbFCA基因。进一步通过构建HbFCA真核过表达载体pXCS-HbFCA-HAStrep,并转化拟南芥发现,转基因植株35 d后已经抽薹开花,野生型对照未开花,HbFCA的过表达能显著促进拟南芥开花。Flowering control locus A（FCA） is an important regulation gene in plant flowering autonomous pathway,it has been proved to be the conservative gene for promoting flowering.In this study,a conserved fragment with 434 bp was obtained from the methylation filtration library in Hevea brasiliensis,based on which the HbFCA gene was cloned successfully by RACE technique.The eukaryotic overexpression vector pXCS-HbFCA-HAStrep was constructed and transformed into Arabidopsis thaliana.The results showed that the transgenic plants flowered after 35 days planting,but the wild type didn＇t bloom,which revealed that the overexpression of HbFCA could promote flowering in Arabidopsis significantly.

关 键 词：橡胶树 HbFCA 转基因 RT-PCR 

分 类 号：S794.1[农业科学—林木遗传育种] Q785[农业科学—林学]