法舒地尔对新生大鼠缺氧缺血性脑损伤后脑组织Nogo髓鞘相关蛋白及NF200表达的影响  

Effects of Fasudil on the expression of Nogo-A and NF200 in neonatal hypoxic-ischemic brain damage rats

作　　者：孟庆云[1] 李丽[1] 邵巍[2] 李慧玲[1] 顾镜月[1] 

机构地区：[1]佳木斯大学附属第一医院儿科,154002 [2]牡丹江医学院附属医院儿内科

出　　处：《中华实用儿科临床杂志》2014年第15期1183-1186,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：黑龙江省卫生厅科研课题（2012-195）

摘　　要：目的 探讨法舒地尔对缺氧缺血性脑损伤（HIBD）大鼠Nogo髓鞘相关蛋白（Nogo-A）及NF200表达的影响.方法 将120只新生7日龄Wistar大鼠按随机数字表法分假手术组（40只）、HIBD组（40只）和法舒地尔治疗组（40只）,假手术组只做颈总动脉分离后不结扎,直接缝合切口不做缺氧处理,HIBD组造模后腹腔注射9 g/L盐水,法舒地尔组腹腔注射法舒地尔（10 mg/kg）.分别于术后6h、12 h、24 h、72 h和7d断颈处死大鼠.HE染色镜下观察各组大鼠脑组织病理变化.免疫组织化学法检测脑组织Nogo-A和NF200表达.结果 1.肉眼观察：假手术组两侧大脑半球对称;HIBD组随时间延长脑组织水肿加重,可见大脑半球坏死灶;法舒地尔组脑组织水肿减轻.2.HE染色：假手术组脑组织结构形态正常;HIBD组可见细胞肿胀,核固缩、裂解,炎性细胞增多;法舒地尔组神经细胞水肿及核固缩数量减少.3.免疫组织化学：假手术组Nogo-A可见少量表达;HIBD组Nogo-A阳性细胞12h开始升高,24 h达峰值,随后表达逐渐降低;法舒地尔组除6h外,各时间点的表达均低于HIBD组（P ＜0.01）;HIBD组和法舒地尔组阳性细胞数多于假手术组（P＜0.01）.假手术组NF200可见微量表达.HIBD组NF200阳性细胞6h出现表达,12h开始下降,24 h降到最低,此后逐渐升高.法舒地尔组各时间NF200的表达均高于HIBD组（P＜0.01）,HIBD组和法舒地尔组NF200多于假手术组（P＜0.01）.结论 法舒地尔可能通过降低Nogo-A的表达和升高NF200的表达促进新生大鼠HIBD后神经细胞轴突的再生和修复.Objective To study the effects of Fasudil on expression of Nogo-A and NF200 in neonatal hypoxic-ischemic brain damage（HIBD） rats.Methods One hundred and twenty 7-day-old Wistar rats were divided into 3 groups with random number table：Sham operation group （n =40）,HIBD group （n =40） and Fasudil group （n =40）.Sham group only separated from the common carotid artery,without ligation,direct suture the incision does not do hypoxia; HIBD group were injected with saline; Fasudil group was injected with fasudil（10 mg/kg）.The rats were killed at 6 h,12 h,24 h,72 h,7 d,after administration.The pathological changes were observed by means of HE.The expression of Nogo-A and NF200 was studied with immunohistochemical staining.Results 1.Naked eye observation：Sham group bilateral symmetrical cerebral hemispheres; HIBD group of brain edema aggravated,the visible hemisphere focal necrosis; fasudil treatment group of edema than HIBD group ease.2.HE stain：the structure and shape of brain in Sham operation group were normal.In HIBD group,the cells became edema,karyopyknosis,lyse,and the inflammatory cells became more.The number of edema cells and karyopyknosis decreased in Fasudil group.3.Immunohistochemical stain：there were less expressions of Nogo-A in Sham operation group.It increased slightly after 12 h in HIBD group but decreased later.The expression of Nogo-A in Fasudil group was less than the other two groups at any time except 6 h （P 〈0.01）.There was more expression in HIBD and Fasudil group compared with Sham operation group（P 〈0.01）.NF200 was less expression in Sham operation group.NF200 appeared after 6 h and became less after 12 h.The expression of NF200 was at 24 h and later became more.The expression of NF200 in Fasudil group was more with HIBD group at each different time （P 〈 0.01）.The expressions of NF200 in Fasudil and HIBD group were more compared with Sham operation group.Conclusions Fasudil can rehabilitate the damaged axon and promote nerve regeneration through contro

关 键 词：缺氧缺血性脑损伤 Nogo髓鞘相关蛋白 NF200 法舒地尔 神经再生 

分 类 号：R722.1[医药卫生—儿科]