穿心莲内酯衍生物炎琥宁对大鼠体内白念珠菌生物膜的影响  

Effect of andrographolide derivative Yanhuning on in vivo Candida albicans biofilms in rats

作　　者：施高翔[1,2] 严园园[1,2] 邵菁[1,2] 张梦翔[1,2] 陆克乔 汪天明[1,2] 汪长中[1,2] 

机构地区：[1]安徽中医药大学中西医结合临床学院,安徽合肥230038 [2]安徽省中医药科学院中西医结合研究所,安徽合肥230038

出　　处：《中国中药杂志》2014年第15期2924-2929,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073127);安徽中医药大学自然科研项目(2013zr009;2013RC001)

摘　　要：目的:探讨穿心莲内酯(AG)衍生物炎琥宁(YHN)对大鼠体内白念珠菌生物膜的影响。方法:构建白念珠菌生物膜大鼠皮下导管模型,采用YHN(40,20,10,5,2.5 mg·kg-1)腹腔注射治疗,设氟康唑(FLC)阳性对照组(80 mg·kg-1),7d后计数导管的菌落形成单位(CFU),XTT代谢法评估YHN对体外白念珠菌生物膜的影响;扫描电镜(SEM)观察YHN干预大鼠体内生物膜的形态变化;采用实时荧光定量PCR法检测白念珠菌黏附相关基因ALS1,ALS3,HWP1,EAP1和MP65的表达量。结果:YHN组导管片上CFU明显少于空白组;XTT代谢活性也显示YHN组低于空白组,且呈剂量依赖性;扫描电镜照片显示YHN能显著减少白念珠菌对大鼠体内导管的黏附;qRT-PCR结果显示,YHN可下调ALS1,ALS3,HWP1,EAP1和MP65的表达量。结论:YHN能抑制大鼠体内白念珠菌生物膜。Objective：To investigate the effect of andrographolide derivative Yanhuning（YHN） on Candida albicans biofilms in rats.Method：The rat C.albicans biofilms subcutaneous catheter model was established by intraperitoneally injecting YHN（40,20,10,5,2.5 mg·kg- 1）,with the FLC（80 mg·kg- 1） positive group as the control group.After 7 d,CFU counting and XTT assay were used to evaluate the effect of YHN on C.albicans biofilms in vivo.Scanning electron microscopy（SEM） was applied to observe the morphological changes in rat biofilms intervened by YHN.The real-time fluorescence quantification PCR was adopted to detect expressions of C.albicans adhesion-related genes,such as ALS1,ALS3,HWP1,EAP1 and MP65.Result：The YHN group showed much less CFUs on catheter pieces and lower XTT metabolic activity than the blank group,with dosage dependence.SEM also showed that YHN could obviously decrease C.albicans adhesion on subcutaneous catheters in rats.According to qRT-PCR＇s results,YHN can down-regulate expressions of ALS1,ALS3,HWP1,EAP1 and MP65.Conclusion：YHN could inhibit C.albicans biofilms in rats.

关 键 词：穿心莲内酯 炎琥宁 白念珠菌 生物膜 体内模型 

分 类 号：R285.5[医药卫生—中药学]