吴茱萸次碱对胰岛素抵抗骨骼肌细胞炎症因子表达的影响  

Effects of rutaecarpine on inflammatory cytokines in insulin resistant primary skeletal muscle cells

作　　者：杨建文[1] 聂绪强[1,2] 史海霞[3] 张玉金[1] 张建永[1] 袁野[4] 卞卡[2,5] 

机构地区：[1]遵义医学院药学院,贵州遵义563000 [2]上海中医药大学穆拉德中药现代化研究中心,上海201203 [3]上海交通大学医学院附属第三人民医院,上海201999 [4]四川雅安职业技术学院,四川雅安625000 [5]乔治.华盛顿大学生物化学与分子生物学系,华盛顿20052

出　　处：《中国中药杂志》2014年第15期2930-2935,共6页China Journal of Chinese Materia Medica

基　　金：遵义医学院博士启动基金项目(F-626);上海市教委高校一氧化氮与炎症医学E研究院计划项目(E-04010)

摘　　要：探讨吴茱萸次碱（rutecarpine,Rut）对胰岛素抵抗原代骨骼肌细胞（insulin resistant primary skeletal muscle cells,IR-PSMC）中炎症因子的影响。培养原代大鼠骨骼肌细胞,显微镜下观察细胞形态学变化,MTT法观察Rut对原代骨骼肌细胞增殖的影响。用棕榈酸（PA）诱导建立IR-PSMC细胞,氧化酶-过氧化物酶（GOD-POD）法检测培养基中葡萄糖浓度,并检测Rut对IR-PSMC细胞中炎症因子IL-1,IL-6,TNF-α的影响。原代骨骼肌细胞培养7 d后,细胞长满培养皿,Rut在0～180.0μmol·L-1,对原代骨骼肌细胞增殖无明显影响。用0.6 mmol·L-1PA培养24 h可建立IR-PSMC细胞,Rut在20～180μmol·L-1抑制IR-PSMC细胞中IL-1,IL-6,TNF-α生成,且具良好的量效关系。Rut可能通过抗炎来促进IR-PSMC细胞葡萄糖吸收及改善胰岛素抵抗。It is now well established that inflammation plays an important role in the development of numerous chronic metabolic diseases including insulin resistance（IR） and type 2 diabetes（T2DM）.Skeletal muscle is responsible for 75% of total insulin-dependent glucose uptake;consequently,skeletal muscle IR is considered to be the primary defect of systemic IR development.Our previous study has shown that rutaecarpine（Rut） can benefit blood lipid profile,mitigate inflammation,and improve kidney,liver,pancreas pathology status of T2 DM rats.However,the effects of Rut on inflammatory cytokines in the development of IR-skeletal muscle cells have not been studied.Thus,our objective was to investigate effects of Rut on inflammatory cytokines interleukin（IL）-1,IL-6 and tumor necrosis factor（TNF）-α in insulin resistant primary skeletal muscle cells（IR-PSMC）.Primary cultures of skeletal muscle cells were prepared from 5 neonate SD rats,and the primary rat skeletal muscle cells were identified by cell morphology,effect of rutaecarpine on cell proliferation by MTT assay.IR-PSMC cells were induced by palmitic acid（PA）,the glucose concentration was measured by glucose oxidase and peroxidase（GOD-POD） method.The effects of Rut on inflammatory cytokines IL-1,IL-6 and TNF-αin IR-PSMC cells were tested by enzyme-linked immunosorbent assay（ELISA） kit.The results show that the primary skeletal muscle cells from neonatal rat cultured for 2-4 days,parallel alignment regularly,and cultured for 7 days,cells fused and myotube formed.It was shown that Rut in concentration 0-180.0 μmol·L- 1 possessed no cytotoxic effect towards cultured primary skeletal muscle cells.However,after 24 h exposure to 0.6 mmol·L- 1PA,primary skeletal muscle cells were able to induce a state of insulin resistance.The results obtained indicated significant decrease（P〈0.05 to P〈 0.001） IL-1,IL-6 and TNF-α production by cultured IR-PSMC cells when incubating 24 hours with Rut,beginning from 20 to 180.0 μmol·L- 1.IL-1,IL-6

关 键 词：原代骨骼肌细胞 吴茱萸次碱 胰岛素抵抗 炎症因子 葡萄糖消耗 

分 类 号：R285[医药卫生—中药学]