金雀异黄素对子宫内膜异位症大鼠模型中ERα、ERβ表达的影响  

Effect of genistein on expression levels of ERα and ERβ in rat model of endometriosis

作　　者：朴云鹤[1] 彭小星[2] 文兰英[1] 金延泽[1] 

机构地区：[1]延边大学附属医院妇产科,吉林延吉133000 [2]长春中医药大学附属医院放射线科

出　　处：《中国妇幼保健》2014年第23期3819-3822,共4页Maternal and Child Health Care of China

基　　金：吉林省科技发展计划项目〔20130101172jc〕

摘　　要：目的:探讨金雀异黄素(GEN)对子宫内膜异位症(EMs)大鼠模型中雌激素受体(ER)α、β表达水平的影响。方法:将大鼠分为正常组(A组)和子宫内膜异位症组;子宫内膜异位症组随机分为4组:模型组(B组)、GEN低剂量组(C组)、GEN中剂量组(D组)和GEN高剂量组(E组)。测量血清中雌二醇(E2)的含量,通过RT-PCR方法检测种植物中ERα、ERβ在mRNA水平上的表达,免疫组化法观察异位内膜组织中ERα、ERβ的表达。结果:与治疗前比较,血清中E2的含量差异无统计学意义(P>0.05);高剂量GEN组子宫内膜异位模型中种植物组织中ERα、ERβmRNA水平下降;种植物中ERα的表达在各组之间无明显差异,但GEN中剂量和GEN高剂量治疗组中种植物的ERβ蛋白表达较模型组明显下降(P<0.01)。结论:GEN对EMs的抑制作用可能更趋向于ERβ选择性拮抗剂的作用。Objective： To explore the eitect of genistein on expression levels oi estrogen receptor （ER） α and ERβ m rat model ot endometriosis. Methods： The rats were divided into normal group （group A） and endometriosis group; the rats in endometriosis group were randomly divided into model group （group B）, lose -dose genistein group （group C）, middle -dose genistein group （group D） and high - dose genistein group （ group E） . The contents of serum estradiol in the five groups were detected, RT - PCR was used to detect the expression levels of ERα mRNA and ERβ mRNA, immunohistoehemieal method was used to observe the expression levels of ERα and ERβ in endometriosis. Results： There was no statistically significant difference in serum estradiol content between before treatment and after treatment （ P 〉 0.05 ） ; ERα mRNA and ERβ mRNA levels in high - dose genistein group decreased after treatment; there was no statistically signifi- cant difference in ERα expression level among the five groups, but ERβ expression levels in middle - dose genistein group and high - dose genistein group decreased significantly compared with model group （P 〈 0. 01 ） . Conclusion： The inhibiting effect of genistein on endometriosis tend to the effect of ERβ - selective antagonist.

关 键 词：金雀异黄素 子宫内膜异位症 大鼠 雌激素受体 

分 类 号：R711.71[医药卫生—妇产科学]