紫外-可见分光光度法测定米非司酮阴道环含量  

Determination of mifepristone in drug loaded vaginal ring by ultraviolet and visible spectrophotometry

作　　者：段雪艳[1] 王介东[1] 宁美英[1] 

机构地区：[1]国家人口计生委科学技术研究所,北京100081

出　　处：《中国计划生育学杂志》2014年第8期547-549,共3页Chinese Journal of Family Planning

基　　金："十二五"国家科技支撑计划课题(2012BAI31B00)

摘　　要：目的：建立一种紫外-可见分光光度法,用于阴道避孕环内米非司酮的含量测定。方法：将米非司酮阴道避孕环切成小碎块,加入甲醇,连续进行12h磁力搅拌,得到米非司酮阴道避孕环的提取液。然后以甲醇为空白对照,在310nm波长处,采用紫外-可见分光光度法测定米非司酮阴道避孕环提取液的吸光度,并通过标准对照法计算载药阴道避孕环中米非司酮的含量。结果：米非司酮在5.0～15.0μg/ml范围内具有良好的线性关系,相关系数r=0.9999,平均回收率为（100.23±0.38）%,精密度和稳定性的相对标准偏差均〈2%。结论：该方法简便、快速和准确,重现性好,可用于米非司酮阴道避孕环的含量测定。Objective： To develop an ultraviolet and visible spectrophotometry （UV-Vis） method for the determination of mifepristone in vaginal rings. Methods.. The vaginal rings were cut into small parts and mifepristone was extracted by methanol from mifepristone-loaded vaginal rings under the condition of magnetic stirring for 12 h. The extraction sample was detected at 310 nm by UV-Vis, using methanol as blank, and external standard method was used for quantification. Results： A good linear relationship for mifepristone was obtained within the range of 5.0-15.0μg/ml （r = 0.9999）. The average recovery was （100.23±0.38）%. The relative standard deviations of precision and stability were both less than 2 %. Conclusion： This method is simple, rapid, accurate and reliable with a good reproducibility. And it is suitable for the determination of mifepristone in vaginal rings.

关 键 词：米非司酮 阴道避孕环 含量测定 紫外-可见分光光度法 

分 类 号：R927[医药卫生—药学] O657.32[理学—分析化学]