宁动颗粒对抽动秽语综合征模型大鼠纹状体5-羟色胺代谢及其受体的影响  

Effect of ningdong granule on 5-hydroxytryptamine metabolism and 5-HT 2AR of tourette’s syndrome rat models

作　　者：赵林[1] 齐芳华[1] 王志学[1] 吕红[2] 李继君[3] 李安源[1] 

机构地区：[1]山东大学附属省立医院中西医结合科,济南250021 [2]滨州医学院中医学教研室,264003 [3]上海交通大学医学院附属儿童医学中心,200127

出　　处：《国际中医中药杂志》2014年第8期715-718,共4页International Journal of Traditional Chinese Medicine

基　　金：基金项目：国家自然科学基金（项目编号：81273798,81202724）;山东省自然科学基金（项目编号：ZR2012HM030）;山东省中医药科技发展计划项目（项目编号：2013ZDZK.085）;山东省科技发展计划项目（项目编号：2011GSF11903）;中国博士后科学基金项目（项目编号：2014M551924）

摘　　要：目的：探讨宁动颗粒对抽动-秽语综合征（TS）模型大鼠纹状体5-羟色胺（5-HT）代谢及其受体5-HT2A受体（5-HT2AR）的影响及其作用机制。方法将64只大鼠按照随机数字表法随机分为空白对照组、模型组、宁动颗粒低剂量组、宁动颗粒高剂量组各16只，通过腹腔注射阿朴吗啡（Apo）建立TS模型。宁动颗粒低、高剂量组分别给予宁动颗粒1.8、3.6 g/（kg？d）；正常对照组及模型组分别给予等量蒸馏水。药物干预12周后，取大鼠纹状体，采用免疫组化法检测纹状体5HT2AR表达，酶联免疫吸附法测定大鼠纹状体5-HT、5-羟吲哚乙酸（5-HIAA）含量。结果模型组大鼠纹状体5-HT、5-HT2AR蛋白表达升高[分别为（1.98±0.14）ng/ml、（143.10±8.01）]，与空白对照组[（1.78±0.10）ng/ml、（127.43±6.72）]比较，差异有统计学意义（P＜0.01或0.05）。宁动颗粒高剂量可上调大鼠纹状体5-HT、5-HT2AR蛋白表达[分别为（2.20±0.14）ng/ml、（166.33±9.21）]，下调5-HIAA表达[（0.58±0.08）ng/ml]，与模型组比较差异有统计学意义（P＜0.01或0.05）。结论 Apo诱导的TS大鼠纹状体存在5-HT递质系统功能增强，但增强功能较弱；宁动颗粒可抑制5-HT代谢，上调TS大鼠纹状体内5-HT2AR蛋白表达和5-HT含量，改善纹状体内5-HT递质系统功能活动的相对不足。Objective To investigate the effect of Ningdong granule on 5-hydroxytryptamine metabolism and 5-HT2AR of Tourette’s Syndrome（TS） rat models. Methods 32 SD rats were divided into 4 groups randomly, the control group, the model （Apo） group, the low dosage NDG （NGL） group, and the high dosage （NGH） group. TS rat models were induced by intraperitoneal injection （i.p.） with Apo （2 mg/kg） in the experimental groups last 4 weeks. Rats in the control group were injected with normal saline （0.9%） （3 ml/kg, i.p.）. Then, the rats were treated by intragastric administration （i.g.） with ND granular at 1.8g/（kg？d）in NGL group, with ND granular at 3.6 g/（kg？d）in NGH group, with distilled water at equal Volume in Control group and Apo group respectively for 12 successive weeks. Analysis the striatum 5-HT2AR protein expression by Immunohistochemical method;Determination of the content of 5-HT and 5-HIAA in striatum by Enzyme linked immunosorbent assay （ELISA）. Results The level of 5-HT and 5-HT2AR protein expression in the model group [（1.98±0.14）ng/ml and（143.10±8.01）], compared with the control group[（1.78±0.10）ng/ml and（127.43±6.72）], was up-regulated （P〈0.01 or 0.05）. The levels of 5-HT and 5-HT2AR protein expression in NGH group[（2.20±0.14）ng/ml and（166.33±9.21）ng/ml] were further up-regulated, and the levels of＆amp;nbsp;5-HIAA in NDH group [（0.58±0.08） ng/ml] became lower, compared with the control group（P＆lt;0.01 or 0.05）. Conclusion Activity of 5-HT system enhanced in Apo-induced TS rat model. However, the enhanced activity may still be relatively insufficient. The mechanism of ND granulear treating TS was associated with depression of the 5-HT metabolism, up-regulation expression of 5-HT2AR, and improving the activity insufficient of 5-HT system.

关 键 词：宁动颗粒 抽动-秽语综合征 5-羟色胺 5-HT2A受体 

分 类 号：R3416[医药卫生—基础医学]