四磨汤对寒冷束缚应激小鼠脑肠肽NOS的影响  

Effects of Simo Decoction on Ghrelin Nos in Cold-Constraint Stressed Mice

作　　者：蔡莹[1] 蔺晓源[2] 蔡光先[2] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学省部共建中医内科学教育部重点实验室,湖南长沙410007

出　　处：《中华中医药学刊》2014年第8期1877-1879,I0006,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究计划(973计划)项目(2009CB523002)

摘　　要：目的:探讨四磨汤对寒冷束缚应激小鼠脑肠肽NOS的影响。方法:动物随机分组,采用寒冷束缚等多种刺激方法,制备胃肠功能紊乱小鼠模型,分别给予蒸馏水、得舒特和四磨汤口服液治疗;免疫组织化学法观察小鼠脑内NOS的阳性表达,用RT-PCR法检测结肠组织NOS mRNA表达。结果:模型组小鼠NOS阳性表达减少,平均OD值与正常组比较差异有显著性统计学意义(P<0.01);四磨汤组NOS表达增加,与模型组比较有统计学差异(P<0.05)。四磨汤可调控模型小鼠结肠组织Nos mRNA,与模型组比较差异有显著性统计学意义(P<0.01)。结论:四磨汤调节脑肠肽NOS的表达可能是其治疗功能性胃肠病的作用机制之一。Objective：To study the effects of Simo Decoction on expression of NOS in cold - constraint stressed mice. Methods：Gastrointestinal dysfunction model was established by exerting the factors of cold - constraint stimulation. And then they were randomly treated with distilled water, Dieetel and SD. The expressions of NOS in brain tissue were meas- ured by immunohistoehemieal staining. The expressions of NOS in intestine were measured by RT - PCR. Result ： The ex- pressions of NOS in brain tissue of model group was decreased significantly compared with that of the normal group （ P 〈 0. 01 ）. The SD could increase the expression of NOS in brain tissue. The average optical density of immune positive result had significant differences compared with that of the model group （ P 〈 0.05 ）. The SD could regulate the expression of NOS mRNA ,which had significant differences compared with that of the model group （P〈 0.01 ）. Conclusion：Simo Decoction appears potential to regulate the expression of NOS in cold - constraint stressed mice, which is one of possible mechanism to treat FGIDs.

关 键 词：四磨汤 寒冷束缚应激 功能性胃肠病 NOS 

分 类 号：R285.5[医药卫生—中药学]