续命汤对局灶性脑缺血中风大鼠紧密连接相关蛋白(Zo-1)和水通道蛋白4 (AQP4) mRNA表达的影响  

Effect of Xuming Decoction on Tight Junction-associated Protein( Zo-1)and Aquaporin 4( AQP4) mRNA Expression in Focal Cerebral Ischemic Stroke Rats

作　　者：周天梅[1] 佟丽妍[1] 徐焕凤 

机构地区：[1]杭州市中医院,浙江杭州310007 [2]石家庄长征医院,河北石家庄050021

出　　处：《中华中医药学刊》2014年第8期1983-1986,I0022,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：杭州市医药卫生科技计划项目(2010B032)

摘　　要：目的:观察续命汤对局灶性脑缺血中风大鼠紧密连接相关蛋白(Zo-1)和水通道蛋白4(AQP4)mRNA表达的影响。方法:将清洁级SD大鼠分为分假手术组、模型组、尼莫地平组、续命汤低剂量组、中剂量组、高剂量组6组,通过栓线法制备局灶性脑缺血中风大鼠模型,观察并记录SD大鼠造模后神经功能评分、免疫组化法检测脑缺血大鼠脑组织紧密连接相关蛋白(Zo-1)和水通道蛋白4(AQP4)mRNA表达。结果:造模后大鼠神经行为学评分显著升高,Zo-1蛋白阳性表达降低,AQP4 mRNA的表达增加,续命汤各治疗组能拮抗以上作用,以续命汤中、高剂量组为显著,其疗效优于或与尼莫地平组相当。结论:续命汤能减少Zo-1蛋白的损失,维持紧密连接复合体结构的完整性,抑制AQP4 mRNA的过度释放与表达,减轻脑缺血和水肿,进而实现保护血脑屏障的作用。Objective ： To observe effect of Xuming Decoction on the tight junction - associated protein （ Zo - 1 ） and aquaporin 4（AQP4） mRNA expressive of focal cerebral ischemic stroke rats. Methods ：The SD rats of clean grade were divided into six groups, which were sham operation group, model group, nimodipine group, Xuming Decoction of low dose group,middle dose group, and high dose group. The models of focal cerebral ischemic stroke rats were prepared by using thread embolism method. Neurological score, expression of tight junction - associated protein （ Zo - 1 ） and aquaporin 4 （AQP4） mRNA in cerebral ischemic rat brain detected by immunohistochemical method were observed and recorded after SD rats modeling. Results ：The neurological score was significantly higher. The expression of Zo - 1 protein was decreased and the expression of AQP4mRNA was increased after modeling in rats, while all Xuming Decoction treatment groups can antagonize these effects,in which the Xuming Decoction of middle dose group and high dose group effects were obvious,and the curative effects were superior to or equivalent with Nimodipine group. Conclusion：Xuming Decoction plays an important roles in reducing the loss of Zo - 1 protein, maintaining the integrity of tight junction complex structure,inhibiting the excessive release and expression of AQP4 and alleviating cerebral ischemia and edema to protect the blood - brain barrier.

关 键 词：续命汤 局灶性脑缺血 紧密连接相关蛋白 水通道蛋白4 

分 类 号：R285.5[医药卫生—中药学]