湘西黄牛IGF–IR基因的群体遗传多态性及其与生长性状的关联分析  

Population genetic polymorphism of IGF–IR gene and its associations with growth traits in Xiangxi cattle

作　　者：王兴平[1,2] 罗仍卓么[1,2] 欧阳助姣 李峰[1,2] 李娜[1,2] 王京仁[1,2] 

机构地区：[1]湖南文理学院生命科学学院,湖南常德415000 [2]湖南省高校动物学重点实验室,湖南常德415000

出　　处：《湖南农业大学学报（自然科学版）》2014年第4期389-394,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技计划项目(2010NK3043);湖南文理学院生命科学学院省级平台开放课题(SKYPT201207);湖南文理学院博士启动项目(BSQD1002);湖南省动物学重点学科建设项目

摘　　要：为探讨湘西黄牛的分子遗传特征,寻找与湘西黄牛生长性状相关的分子标记,用PCR和基因测序技术检测了湘西黄牛IGF–IR基因部分序列的碱基突变,并采用PCR–RFLP方法进行了群体遗传多态性分析。结果表明:PCR扩增序列为616 bp,与预期结果相符,测序的序列中存在34个单核苷酸多态性(SNPs)位点,其中1个SNP已有报道(g.551G>T),33个SNPs为首次发现;g.551G>T位点在湘西黄牛群体中存在A和B 2个等位基因,A为优势等位基因,检测到AA、AB和BB 3种基因型;该位点在湘西黄牛群体中呈中度多态,达到了Hardy–Weinberg平衡状态(P>0.05);将湘西黄牛IGF–IR基因的多态性与其生长性状进行关联分析,结果表明,g.551G>T位点对湘西黄牛的体高、体长、胸围和体重无显著影响(P>0.05)。Aiming at investigating molecular genetic characters and searching molecular marker-related growth traits in Xiangxi cattle, the mutations of bovine 1GF-1R gene partial sequence were detected by PCR and gene sequencing, and PCR-RFLP was used to analyze population genetic polymorphism of IGF-1R gene. The results showed the expected 616 bp 1GF-IR patial sequence PCR products was obtained, sequence analysis indicated that 1GF-IR gene in Xiangxi cattle contained a total of 34 SNPs, in which, one SNP had been reported in previous study, while 33 SNPs were firstly found in this study. Two alleles （A and B） and three genotypes （AA, AB and BB） of g.551G〉T locus in 1GF-IR gene were detected in Xiangxi cattle, A allele was dominate allele, which were in moderate polymorphism, and fitted in Hardy-Weinberg equilibrium （P〉0.05） in Xiangxi cattle population. The result of the association between polymorphism and growth traits indicated that g.551G〉T locus had no significant influence on height, body length, heart girth and body weight of Xiangxi cattle （P〉0.05）.

关 键 词：湘西黄牛 IGF–IR基因 群体遗传多态性 生长性状 

分 类 号：S823.81[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]