异丙酚对胎鼠离体海马神经元增殖的影响  

Effects of propofol on proliferation of hippocampal neurons in fetal rats in vitro

作　　者：钟玉玲[1] 韦祎 梁羽冰[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021

出　　处：《中华麻醉学杂志》2014年第5期552-554,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81060277,81373498）;广西科学研究与技术开发计划项目（桂科攻1355005-4-2）;广西高校科学技术研究项目（2013ZD014）

摘　　要：目的 评价异丙酚对胎鼠离体海马神经元增殖的影响.方法 孕16～ 18 d SD大鼠,拉颈处死,剖腹取胎鼠,分离海马神经元.将神经元接种于培养板中,培养至第9天,采用随机数字表法,将其分为7组（n=36）：对照组（C组）、脂肪乳剂组（I组）、异丙酚0.1 μmol/L组（P1组）、异丙酚1.0μmol/L组（P2组）、异丙酚10.0 μmol/L组（P3组）、异丙酚100.0 μmol/L组（P4组）和异丙酚1 000.0μmol/L组（P5组）.C组不做任何处理;I组培养液中加入10％脂肪乳剂,终浓度为100 μmol/L;P1组、P2组、P3组、P4组和P5组培养液中加入异丙酚,终浓度分别为0.1、1.0、10.0、100.0、1 000.0 μmol/L,继续孵育3h.分别于异丙酚孵育结束后12、24、36、48、60和72 h时采用MTT法测定海马神经元增殖水平.结果 与C组比较,P1组、P2组、P3组、P4组和P5组海马神经元增殖水平降低（P＜0.05）,I组海马神经元增殖水平差异无统计学意义（P＞0.05）;与P1组比较,P2组、P3组和P4组海马神经元增殖水平差异无统计学意义（P＞0.05）,P5组海马神经元增殖水平降低（P＜0.05）.结论 异丙酚可抑制胎鼠离体海马神经元增殖.Objective To evaluate the effects of propofol on the proliferation of hippocampal neurons in fetal rats in vitro.Methods Pregnant Sprague-Dawley rats at 16-18 days of gestation,were sacrificed and the fetal rats were taken out from the abdominal cavity.The hippocampal neurons of the fetal rats were isolated and seeded in culture plates.After being cultured for 9 days,the neurons were divided into 7 groups using a random number table（n =36 each）：control group （C group）,intralipid group （I group） and propofol 0.1,1.0,10.0,100.0,1 000.0 μmol/L groups （P1-5 groups）.In group I,10% intralipid was added to the culture media until the final concentration reached 100 μmol/L.In P1-5 groups,propofol was added to the culture media until the final concentration reached 0.1,1.0,10.0,100.0 and 1 000.0 μmol/L,respectively.The neurons were then incubated for 3 h.The proliferation of hippocampal neurons was determined by MTT assay at 12,24,36,48,60 and 72 h after the end of incubation with propofol.Results Compared with group C,the proliferation of hippocampal neurons was significantly decreased in P1-5 groups （P 〈 0.05）,while no significant change was found in group I （P 〉 0.05）.Compared with group P1,the proliferation of hippocampal neurons was significantly decreased in group P5 （P 〈 0.05）,while no significant change was found in P2-4 groups （P 〉 0.05）.Conclusion Propofol can decrease the proliferation of hippocampal neurons in fetal rats in vitro.

关 键 词：二异丙酚 胎儿 海马 神经元 细胞增殖 

分 类 号：R614[医药卫生—麻醉学]