富氢液对大鼠切口痛-瑞芬太尼诱发痛觉过敏的影响  

Effects of hydrogen-rich saline on incisional pain-remifentanil-induced hyperalgesia in rats

作　　者：张麟临 舒瑞辰 王春艳[1] 李楠[1] 王海云[1] 于泳浩[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2014年第5期559-562,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81371245,30972847,81071059,81100984）;天津市科技支撑计划重点项目（12ZCZDSY03000）;天津市卫生局科技基金（09KZ106）

摘　　要：目的 评价富氢液对大鼠切口痛-瑞芬太尼诱发痛觉过敏的影响.方法 尾静脉置管成功的雄性SD大鼠32只,2～3月龄,体重240 ～ 260 g,采用随机数字表法分为4组（n=8）：对照组（C组）、瑞芬太尼＋切口痛组（R＋I组）和不同剂量富氢液组（H1和H2组）.R＋I组、H1组和H2组建立大鼠切口痛模型,同时静脉输注瑞芬太尼1 μg·kg-1·min-1 60 min;C组静脉输注等容量生理盐水60min.H1组和H2组于切口痛模型建立前10 min分别腹腔注射富氢液5和10 ml/kg.分别于输注瑞芬太尼前24 h、输注停止后2、6、24和48 h（T0-T4）时,测定大鼠机械刺激缩足阈（PWT）和热刺激缩足潜伏期（PWL）.痛阈测定结束后处死大鼠,取脊髓L4-6节段,采用Western blot法测定脊髓神经元总蛋白（t）及膜蛋白（m）含R1亚基的NMDA受体（NR1）和含2B亚基的NMDA受体（NR2B）的表达,并计算mNR1/tNR1和mNR2B/tNR2B的比值.结果 与C组比较,R＋I组、H1组和H2组PWT降低,PWL缩短,mNR1和mNR2B、tNR1和tNR2B的表达上调,mNR1/tNR1和mNR2B/tNR2B的比值升高（P＜0.05）.与R＋I组比较,H1组和H2组PWT升高,PWL延长,mNR1和mNR2B的表达下调,mNR1/tNR1和mNR2B/tNR2B的比值降低（P＜0.05）.与H1组比较,H2组PWT升高,PWL延长,mNR1和mNR2B的表达下调,mNR1/tNR1和mNR2B/tNR2B的比值降低（P＜0.05）.结论 富氢液可减轻大鼠切口痛-瑞芬太尼诱发痛觉过敏,其机制可能与抑制脊髓神经元NR1和NR2B从胞浆向胞膜的转运有关.Objective To evaluate the effects of hydrogen-rich saline on incisional pain-remifentanil-induced hyperalgesia in rats.Methods Thirty-two male Sprague-Dawley rats,aged 2-3 months,weighing 240-260 g,in which the catheter was successfully inserted into the caudal vein,were randomly divided into 4 groups （n =8 each） using a random number table：control gourp （group C）,remifentanil ＋ incisional pain group （group R ＋ I） and different doses of hydrogen-rich saline groups （H1 and H2 groups）.A l-cm longitudinal incision was made through skin,fascia and muscle of the plantar aspect of the left hindpaw in chloral hydrate-anesthetized rats.Remifentanil 1 μg· kg-1 · min-1 was infused intravenously for 60 min sarting from beginning of establishment of incisional pain model in R ＋ I,H1 and H2 groups.The equal volume of normal saline was infused intravenóusly for 60 rin instead of remifentanil group C.Hydrogen-rich saline 5 and 10 ml/kg were injected intraperitoneally at 10 min before establishment of incisional pain model in H1 and H2 groups,respectively.Paw withdrawal threshold （PWT） to yon Frey hair stimulation and paw withdrawal latency （PWL） to thermal stimulation were measured at 24 h before remifentanil infusion and 2,6,24 and 48 h after remifentanil infusion （T0-T4）.The rats were sacrificed after measuremnt of pain threshold,and L4-6 segments of the spinal cord were removed for determination of the expression of R1 and 2B subunits-containing N-methyl-D-aspartate receptors （NR1 and NR2B） in total and membrane proteins by Western blot.The ratio between the expression of NR1 in membrane protein and in total protein （mNR1/tNR1） and NR2B in membrane protein and in total protein （mNR2B/tNR2B） was calculated.Results Compared with group C,PWT was significantly decreased,PWL was shortened,the expression of mNR1,mNR2B,tNR1 and tNR2B was up-regulated,and the ratios of mNR1/tNR1 and mNR2B/tNR2B were increased in R ＋ I,H1 and H2 groups.Compared with group R ＋ I,PWT was significa

关 键 词：氢 痛觉过敏 哌啶类 疼痛 手术后 

分 类 号：R614[医药卫生—麻醉学]