乌司他丁对应激性胃溃疡大鼠Th1/Th2细胞平衡的影响  

Effect of ulinastatin on Th1/Th2 cell balance in a rat model of stress-induced gastric ulcer

作　　者：杨威[1] 许德奖[1] 赵刚[2] 赵国栋[1] 

机构地区：[1]广东省医学科学院广东省人民医院麻醉科,广州市510080 [2]广东省医学科学院广东省人民医院普外科,广州市510080

出　　处：《中华麻醉学杂志》2014年第5期624-626,共3页Chinese Journal of Anesthesiology

基　　金：广东省科技计划项目（2012B031800161,2011B31800387）

摘　　要：目的 评价乌司他丁对应激性胃溃疡大鼠Th1/Th2细胞平衡的影响.方法 SPF级雄性SD大鼠24只,6～8周龄,体重180 ～ 220 g,采用随机数字表法分为3组（n=8）：对照组（C组）、应激性胃溃疡组（GU组）和乌司他丁组（U组）.采用束缚浸水应激3.5h的方法制备大鼠应激性胃溃疡模型,浸水前10 min,GU组经尾静脉注射生理盐水0.4ml,U组经尾静脉注射乌司他丁10万U/kg.于模型制备完成后即刻,麻醉下开腹,显微镜下评价胃粘膜损伤程度,记录胃溃疡指数（UI）,并观察胃粘膜病理学结果;取脾组织,采用PCR法测定脾组织T-bet、Gata-3、干扰素-γ（IFN-γ）、IL-4的mRNA表达.结果 与C组比较,GU组T-bet mRNA表达下调,Gata-3 mRNA表达上调,T-bet/Gata-3比值降低,UI升高（P＜0.05）,IFN-γ和IL-4的mRNA表达差异无统计学意义（P＞0.05）,U组UI升高（P＜0.05）,其余各指标差异无统计学意义（P＞0.05）.与GU组比较,U组T-bet/Gata-3比值升高,UI降低（P＜0.05）,T-bet、Gata-3、IFN-γ、IL-4的mRNA表达差异无统计学意义（P＞0.05）.U组胃粘膜病理学损伤较GU组减轻.结论 乌司他丁减轻大鼠应激性胃溃疡的机制可能与纠正Th1/Th2细胞失衡状态有关.Objective To evaluate the effect of ulinastatin on Th1/Th2 cell balance in a rat model of stress-induced gastric ulcer （GU）.Methods Twenty-four male SPF Sprague-Dawley rats,aged 6-8 weeks,weighing 180-220 g,were randomly divided into 3 groups （n =8 each） using a random number table：control group （group C）,stress-induced GU group （group GU） and ulinastatin group （group U）.The rats were subjected to water-immersion and restraint stress for 3.5 h to establish GU model in S and U groups.At 10 min before water-immersion,normal saline 0.4 ml and ulinastatin 100 000 U/kg were injected via the tail vein in GU and U groups,respectively.After GU model was established,the abdomen was opened after anesthesia.The whole stomach was exposed,and the gastric mucosal damage was evaluated under microscope,ulcer index （UI） was recorded,and the pathological changes of the gastric mucous membrane were observed.Spleen samples were obtained to detect T-bet,Gata-3,interferon-γ （IFN-γ）,and interleukin-4 （IL-4） mRNA expression by RT-PCR.The ratio of T-bet mRNA expression/Gata-3 mRNA expression （T-bet/Gata-3） was calculated.Results Compared with group C,T-bet mRNA expression was significantly down-regulated,Gata-3 mRNA expression was up-regulated,T-bet/Gata-3 ratio was decreased,UI was increased,and no significant changes were found in IFN-γ and IL-4 mRNA expression in GU group,and UI was increased,and no significant changes were found in the other parameters in group U.Compared with group GU,the ratio of T-bet/Gata-3 was significantly increased,and UI was decreased,and no significant changes were found in T-bet,Gata-3,IFN-γ,IL-4 mRNA expression in group U.The gastric mucosal damage was significantly mitigated in GU group as compared with group U.Conclusion The mechanism by which ulinastatin mitigates stress-induced GU may be related to correction of Th1/Th2 cell imbalance in rats.

关 键 词：胰蛋白酶抑制剂 胃溃疡 应激 T淋巴细胞 辅助诱导 

分 类 号：R965[医药卫生—药理学]