新疆野生阿魏侧耳遗传多样性的RAPD分析  

RAPD Analysis of Natural Populations of Pleurotus ferulae in Xinjiang

作　　者：贾培松[1] 郝敬喆[1] 努尔孜亚[1] 贾文捷[1] 魏鹏[1] 

机构地区：[1]新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2014年第6期1131-1136,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重大专项(201130104-2);新疆农科院青年基金项目(xjnkq-2013006);新疆维吾尔自治区公益性科研院所基本科研业务经费资助(KY2014035)

摘　　要：[目的]揭示新疆野生阿魏侧耳种质资源的遗传多样性,为进一步驯化和保护阿魏侧耳野生资源提供基础材料和科学依据.[方法]利用RAPD分子标记技术分析32个阿魏菇菌株的遗传多样性.[结果]从28条随机引物中筛选到6条引物,共扩增出84条DNA条带,多态性条带83条,多态比率为98.8％.供试菌株两两间的相异系数从0.104 2-0.748 5,D=0.60时,32个供试菌株分为5个类群.[结论]新疆野生阿魏侧耳种质资源存在一定的遗传多样性,具有较好的驯化选育潜力和保育价值.[ Objective] The purpose of this study is to reveal the genetic diversity of wild resources ot.ferulae in Xinjiang, which would provide basic materials and scientific basis for the further breeding and pro-tecting of P. ferulae. [ Method] The genetic diversity of 32 P. ferulae strains were analyzed by RAPD molecu-lar marker technology. [ Result ]6 primers were selected from 28 random primers, and 84 DNA bands wereobtained, among 83 bands were polymorphie bands which occupied 98.8% of the total amplified bands. Clus-ter analysis showed that the 32 strains of P. ferulae could be divided into 5 groups at the level of D = 0.6. [ Conclusion ] The results showed that the natural populations of P. ferulae in Xinjiang presence of certain ge-netic diversity, and have some value for breeding and conservation.

关 键 词：野生阿魏菇 遗传多样性 聚类分析 

分 类 号：S646[农业科学—蔬菜学]