一测多评法测定鸡骨草叶中3种黄酮碳苷含量  

Simultaneous Determination of Three Flavones C-glycosides in Leaves of Herba Abri by Quantitative Analysis of Multi-components by Single Marker

作　　者：袁旭江[1] 李春阳[1] 张平[1] 

机构地区：[1]广东药学院中药研究开发所,广东广州510006

出　　处：《中药新药与临床药理》2014年第4期493-497,518,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立鸡骨草叶中3种黄酮碳苷含量的一测多评法(QAMS)。方法通过外标法建立并计算鸡骨草叶3种黄酮碳苷的含量(实测值),并以芹菜素-6,8-C-二葡萄糖苷为内标,建立其与芹菜素-6-C-阿拉伯糖-8-C-葡萄糖苷和芹菜素-6-C-葡萄糖-8-C-阿拉伯糖苷的相对校正因子(RCF),以该RCF计算芹菜素-6-C-阿拉伯糖-8-C-葡萄糖苷和芹菜素-6-C-葡萄糖-8-C-阿拉伯糖苷的含量(计算值),并比较计算值与实测值之间的差异,实现一测多评。结果芹菜素-6-C-阿拉伯糖-8-C-葡萄糖苷和芹菜素-6-C-葡萄糖-8-C-阿拉伯糖苷与芹菜素-6,8-C-二葡萄糖苷之间的RCF分别为1.02、1.31,建立的RCF具有良好重现性和稳定性,20批鸡骨草叶中三种黄酮碳苷含量的计算值与实测值的相对标准偏差<3.5%。结论本文建立的QAMS是快速准确可行的,可用于鸡骨草叶的质量评价。Objective To develop a method for the simultaneous determination of three flavones C-glycosides in the leaves of Herba Abri by quantitative analysis of multi-components by single marker （QAMS）. Methods The contentsof three flavones C-glycosides in 20 batches of samples were determined by external standard method （ESM）. Using 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin as the internal reference substance, the relative correction factors （RCF） of 6-C-arabinosyl-8-C-glycopyranosyl apigenin and 6-C-glycopyranosyl-8-C-arabinosyl apigenin to 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin were determined, and their contents were calculated according to the RCF. The method was evaluated by comparing the quantitative results between ESM and QAMS. Results The RCF values of 6-C-arabinosyl-8-C-glycopyranosyl apigenin, 6-C-glycopyranosyl-8-C-arabinosyl apigenin to 6-C- glycopyranosyl-8-C-glycopyranosyl apigenin were 1.02 and 1.31, respectively. The RCF had good stability and reproducibility. No significant differences were found in the quantitative results between ESM and QAMS（RSD 〈 3.5 % ）. Conclusion The established QAMS is a fast, accurate and feasible method for evaluating the quality of the leaves of Herba Abri.

关 键 词：鸡骨草 毛鸡骨草 一测多评 芹菜素-6 8-C-二葡萄糖苷 芹菜素-6-C-阿拉伯糖-8-C-葡萄糖苷 芹菜素-6-C-葡萄糖-8-C-阿拉伯糖苷 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]