舒脉汤对血管内皮细胞VEGF、bFGF表达的影响及其信号通路  

Effect of Shumaitang on VEGF and bFGF expression of vascular endothelial cells and its possible signal pathway

作　　者：戴红艳[1] 何涛[1] 邢明青[1] 刘文琪[1] 吴晓平[1] 管军[1] 

机构地区：[1]山东省青岛市市立医院心内科,266011

出　　处：《临床合理用药杂志》2014年第26期4-5,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的研究舒脉汤对血管内皮细胞生成因子(VEGF)、碱性成纤维细胞生成因子(bFGF)的影响及其可能参与的信号通路。方法原代培养人脐静脉内皮细胞,以舒脉汤刺激内皮细胞,部分给予PI3K抑制剂,LY294002预处理,收集细胞测定VEGF、bFGF基因表达,收集上清液测定VEGF、bFGF蛋白表达。结果舒脉汤可以明显增加人脐静脉内皮细胞VEGF、bFGF基因及蛋白表达,且这一过程可被LY294002所阻断。结论舒脉汤通过上调内皮细胞VEGF、bFGF表达来发挥促血管新生作用,PI3K信号通路参与此过程。Objective To study the effect of Shumaitang on vascular endothelial growth factor（ VEGF） and basic fibroblast growth factor（ bFGF） expression of vascular endothelial cells and its possible signal pathway. Methods Primary cultured human umbilical vein endothelial cells（ HUVEC） were stimulated by Shumaitang,part of them were pretreated by PI3 K inhibitor LY294002. Cells were collected for determination of VEGF,bFGF gene expression; the supernatant was collected for determination of VEGF,bFGF protein expression. Results Shumaitang could increase the gene and protein expression of VEGF and bFGF of vascular endothelial cells significantly,and this effect was blocked by LY294002. Conclusion PI3 K /VEGF and PI3 K / bFGF may take part in the Shumaitang-mediated-angiogenesis.

关 键 词：舒脉汤 VEGF BFGF 

分 类 号：R285[医药卫生—中药学]