毛白杨PtoNAC157基因的克隆与表达分析  

Molecular Cloning and Expression Analysis of PtoNAC157 from Populus tomentosa

作　　者：张杰伟[1] 陈亚娟[1] 丁莉萍[1] 朱丹[1] 苗傲雪 魏建华[1] 王宏芝[1] 

机构地区：[1]北京市农林科学院北京农业生物技术研究中心农业基因资源与生物技术北京市重点实验室,北京100097

出　　处：《植物生理学报》2014年第7期995-1001,共7页Plant Physiology Journal

基　　金：国家“863”项目(2011AA100201);国家“973”项目(2012CB114501)

摘　　要：NAC是植物特有的一类转录因子,在植物生长发育和抗逆过程中有重要作用。本研究从毛白杨中克隆出一个NAC转录因子基因,并根据同源性分析将其命名为Pto NAC157,开放阅读框为942 bp,能编码一个由313个氨基酸残基组成的蛋白质,预测蛋白分子量为35.5 kDa,等电点为7.22。氨基酸同源性分析显示,该蛋白N-端具有保守的NAC结构域。实时荧光定量PCR结果表明:PtoNAC157在毛白杨幼根、茎和叶中均有表达,在茎中的表达量最高。PtoNAC157响应低温、NaCl(300mmol·L-1)、干旱和ABA(200μmol·L-1)胁迫。由此推测该基因在林木次生生长及响应胁迫信号转导过程中发挥作用。NAC family genes encode plant-specific transcription factors that play important roles in plant development regulation and various stresses. In the present study, we isolated and characterized a gene from 6-month-old Populus tomentosa, which had significant homology to PtrNAC157 from P trichocarpa. The gene was designated as PtoNAC157. The gene encoded a 313-amino-acid polypeptide with a predicted molecular mass of 35.5 kDa and isoelectric point of 7.22. Multiple sequence alignments showed that PtoNAC 157 contained the highly conservative NAC domain that was located in the N-terminal region. PtoNAC157 was most abundantly expressed in stem with secondary growth. Moreover, the transcript expression of PtoNAC157 was induced by cold, dehydration, abscisic acid （ABA） and NaCI stresses. These results suggest that PtoNAC157 might play important roles in secondary xylem development and stress signal transduction in P tomentosa.

关 键 词：毛白杨 PtoNAC157 次生生长 胁迫 

分 类 号：S792.117[农业科学—林木遗传育种]