N-乙酰半胱氨酸对胰岛素抵抗大鼠GLP-1、IL-6表达的影响  

Effects of N-acetylcysteine on GLP-1 and IL-6 in high-fat-diet rats with insulin resistance

作　　者：韩文洁 于苏国[1] 崔翔宇 

机构地区：[1]滨州医学院附属医院内分泌科,山东滨州256603 [2]青岛市中心医院胸外科,山东青岛266000

出　　处：《山东大学学报（医学版）》2014年第6期12-16,21,共6页Journal of Shandong University:Health Sciences

摘　　要：目的观察N-乙酰半胱氨酸(NAC)对高脂饮食诱导胰岛素抵抗大鼠血清胰高血糖素样肽-1(GLP-1)水平及肝脏、胰腺组织中白介素-6(IL-6)表达的影响。方法选取健康雄性SD大鼠40只随机分为两组:对照组(NC组,n=10)给予普通饲料喂养;高脂组(HF组,n=30)喂高脂饲料,12周后计算胰岛素敏感指数(ISI)和胰岛素抵抗指数(HOMA-IR),确定30只胰岛素抵抗大鼠模型建立。将HF组随机分为高脂对照组(HFN组,n=15)和高脂+NAC组(HF+NAC组,n=15),HF+NAC组喂饲高脂饲料的同时给予NAC 300 mg/(kg·d)灌胃,NC组和HFN组给予相同体积的生理盐水灌胃。灌胃4周后,内眦静脉丛采血测定大鼠空腹血糖、空腹胰岛素及血脂水平,HE染色光镜下观察肝脏组织学改变,应用酶联免疫吸附法检测血清中GLP-1水平,免疫组化技术检测肝脏、胰腺组织中IL-6的表达。结果 HFN组大鼠胰岛素敏感性下降,糖耐量减低,出现脂肪肝;HF+NAC组大鼠血清GLP-1水平与HFN组相比显著增加(P<0.05),HF+NAC组肝脏、胰腺组织中IL-6的表达明显低于HFN组(P<0.05)。结论 NAC可以提高胰岛素抵抗大鼠的GLP-1水平、降低IL-6蛋白表达活性,从而起到减轻胰岛素抵抗的作用。Objective To investigate the effects of N-acetylcysteine （NAC） on serous glucagon likepeptide-1（GLP-1） and interleukin-6 （ IL-6 ） expressions in the hepatic and pancreas tissues of SD rats which were insulin resistant after high-fat forage.Methods A total of 40 healthy adult male SD rats were randomly divided into two groups： control group （NC group, n=10）, fed with normal diet; high-fat group （HF group, n=30）, fed with fat-rich diet to con-struct models of insulin resistance.After 12 weeks, fasting plasma glucose （ FPG） , fasting insulin （ FIns） , serum lip-ids, insulin sensitivity and insulin resistance index （ HOMA-IR） were detected to confirm the construction of models. The HF group was randomly subdivided into high fat control group （HFN group, n=15）, and high fat＋NAC group （HF＋NAC group, n=15）.Rats in the HF＋NAC group were given NAC 300 mg/（kg· d）by gavage once a day with high-fat diet simultaneously.Rats in NC group and HFN group were given the same volume of physiological saline with the same method.After 4 weeks, blood samples were collected from angula vein to test FPG, FIns and blood lipid levels.Meanwhile, the pathological changes of liver were observed under light microscope after HE staining.The ser-um level of GLP-1 was examined by enzyme-linked immunoassay （ ELISA） .The expression of IL-6 in the hepatic tis-sues and pancreas was detected by immunohis-tochemistry.Results In HFN group, the sensitivity to insulin decreased,＆nbsp;glucose tolerance impaired, and fatty liver occurred.Serous level of GLP-1 significantly increased in HF＋NAC group compared with HFN group （P〈0.05）;the expression of IL-6 in the liver and pancreas tissues in HF＋NAC group was much lower than that of the control group （P〈0.05）.Conclusion NAC would reduce the insulin resistance in high-fat-diet rats by increasing the serous level of GLP-1 while reducing IL-6 protein expression and activity, which has a protective effect on insulin resistance.

关 键 词：N-乙酰半胱氨酸 胰岛素抵抗 胰高血糖素样肽-1 白介素-6 

分 类 号：R587.1[医药卫生—内分泌]