维生素D受体基因ApaI多态性与糖耐量减低的相关性  

Association between vitamin D receptor gene ApaI polymorphism and impaired glucose tolerance

作　　者：侯丽君[1] 邢鹂健[2] 李广红[1] 刘燕[3] 亓文波[4] 

机构地区：[1]泰山医学院附属医院内分泌科,山东泰安271000 [2]泰山医学院诊断学教研室,山东泰安271000 [3]泰安市中心医院老年病科,山东泰安271000 [4]泰安市中心医院内分泌科,山东泰安271000

出　　处：《山东大学学报（医学版）》2014年第6期51-54,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨维生素D受体(VDR)基因ApaI多态性与糖耐量减低(IGT)及25羟基维生素D3(25-OH-VD3)的相关性。方法采用聚合酶链反应限制性片段长度多态性(PCR-RLFP)技术,检测105例IGT(IGT组)和105例健康者(NC组)VDR ApaI基因型,酶联免疫吸附(ELISA)法测血清25-OH-VD3水平,并检测相关临床及生化指标,比较VDR ApaI基因型和等位基因频率分布差异及不同基因型血清25-OH-VD3等相关指标的差异。结果①血清25-OH-VD3水平IGT组较NC组低(22.04±2.37 vs 32.04±2.64),P<0.05。血清25-OH-VD3水平<30 ng/mL占63.8%。②VDR基因ApaI位点基因型和等位基因频率在两组中的分布差异明显(P<0.05),IGT组等位基因a和基因型aa分布频率明显高于NC组。③基因型AA、Aa的25-OH-VD3高于aa(P<0.05)。血清低密度脂蛋白胆固醇(LDL-C)、体质量指数(BMI)、年龄、VDR基因ApaI与IGT发病呈正相关(OR分别为1.832、1.916、1.466、1.812,P<0.05),而与血清25-OH-VD3水平呈负相关(OR=-0.461)。结论维生素D不足较为普遍;VDR基因ApaI等位基因a和基因型aa可能为易发生IGT的危险性遗传标志;血清25-OH-VD3可能与VDR基因ApaI多态性相关。Objective To investigate the association between vitamin D receptor（ VDR） gene ApaI polymorphism and impaired glucose tolerance （IGT） and blood serum 25-dihydroxyvitamin D （25-OH-VD3）.Methods VDR genotype in 105 cases of IGT（IGT group） and 105 normal controls（NC group） was detected with PCR-RLFP; serum 25-OH-VD3 was tested with enzyme linked immunosorbent assay（ ELISA）;relevant clinical and biochemical indexes were also examined.The alleles frequencies and genotype frequencies of VDR ApaI gene were compared between the two groups, and serum 25-OH-VD3 and other indexes were compared between different genotypes.Results Compared with con-trols, subjects with IGT had a markedly lower level of serum 25-OH-VD3 （22.05 ±2.37 vs 32.04 ±2.64）, P〈0.05. Level of serum 25-OH-VD3 lower than 30 ng/mL accounted for 63.8%of all cases.There was significant difference in the distribution of VDR genotype frequencies and alleles frequencies between the two groups （P〈0.05）.The prevalence＆nbsp;of VDR gene genotype frequencies and alleles frequencies in IGT was significantly different from the controls.Serum 25-OH-VD3 was higher in VDR genotype AA and Aa groups than in genotype aa groups （ P〈0.05）.Multivariate logistic regression analysis showed that the levels of low density lipoprotein cholesterol（LDL-C）, BMI, age, VDR gene ApaI presented a positive correlation with IGT, while 25-OH-VD3 exhibited a negative correlation with IGT. Conclusion Vitamin D deficiency is prevalent.Allele a of VDR may be a genetic marker susceptible to IGT.Serum 25-OH-VD3 is associated with VDR gene polymorphism.

关 键 词：糖耐量减低 VDR基因多态性 25羟基维生素D3 

分 类 号：R591.44[医药卫生—内科学]