精子相关抗原9在宫颈癌及宫颈上皮内瘤变中的表达及意义  

Expression and clinical significance of sperm-associated antigen 9 in patients with cervical cancer and cervical intraepithelial neoplasia

作　　者：张璐[1] 颜磊[1] 张辉[1] 张晓晖[1] 李明江[1] 赵兴波[1] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2014年第6期67-71,77,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81202057);山东省自然科学基金(ZR2010HM005)

摘　　要：目的检测精子相关抗原9(SPAG9)在宫颈癌、宫颈上皮内瘤变(CIN)组织中的表达及意义。方法通过免疫组织化学、Western blotting、RT-PCR方法检测正常宫颈组织(对照组,n=20)、CIN组织(CIN组,n=35)及宫颈癌组织(宫颈癌组,n=40)中SPAG9的表达。应用酶联免疫吸附试验(ELISA)测定健康女性、子宫肌瘤患者、CIN患者及宫颈癌患者血清标本中SPAG9浓度。结果①SPAG9的表达:宫颈癌组>CIN组>对照组,各组间差异有统计学意义(P均<0.05);②SPAG9 mRNA和蛋白在宫颈癌组中高表达,在对照组中无表达(P<0.05);③宫颈癌患者的SPAG9血清浓度高于CIN患者、子宫肌瘤患者及健康女性的SPAG9血清浓度(P<0.05),且患者术后的SPAG9血清浓度下降。结论 SPAG9在宫颈癌和CIN组织以及患者血清中表达量均增高,其作为一个新的辅助生物学指标,可为宫颈癌及CIN的早期诊断、术后检测及随诊治疗提供指导意义。Objective To investigate the clinical significance of sperm-associated antigen 9 （ SPAG9） in cervical cancer and cervical intraepithelial neoplasia （ CIN） patients.Methods The expressions of SPAG9 mRNA and protein were evaluated in cervical carcinoma （cervical carcinoma group, n=40）, CIN （CIN group, n=35） and normal cervical tis-sues （ the control group, n=20） by immunohistochemistry, RT-PCR, and Western blotting.SPAG9 concentrations in serum samples from 10 healthy women, 20 women with hysteromyoma, 40 women with CIN, 40 women with cervical carcinoma, and 20 postoperative cervical carcinoma patients were detected by enzyme-linked immunosorbent assay （ ELISA） .Results ①The expression of SPAG9 was gradually increased in the control group, CIN group and cervical carcinomas group （all P〈0.05）;②A significant difference has been found between cervical carcinoma group and the control group by RT-PCR and Western blotting （P〈0.05）;③SPAG9 serum concentrations in cervical carcinoma pa-tients was significantly higher than those in the CIN and hysteromyoma patients and the healthy women （P〈0.05）. Furthermore, a significant decrease of SPAG9 level was detected after operation.Conclusion SPAG9 is positively ex-pressed in cervical carcinoma and CIN.A high humoral response against SPAG9 suggests its key role in early detection, clinical diagnosis and postoperative monitoring of cervical carcinoma and CIN patients.

关 键 词：精子相关抗原9 宫颈癌 宫颈上皮内瘤变 诊断 

分 类 号：R711.75[医药卫生—妇产科学]