PAI-1基因多态性与烧伤脓毒症的相关性  

Correlation between PAI-1 gene polymorphism and sepsis in severely burned patients

作　　者：吴学田[1] 王坤[1] 孙树栋[1] 

机构地区：[1]潍坊市人民医院烧伤科,山东潍坊261041

出　　处：《山东大学学报（医学版）》2014年第6期105-107,112,共4页Journal of Shandong University:Health Sciences

基　　金：2003年潍坊市卫生科技发展计划第20号课题

摘　　要：目的探讨血浆纤溶酶原激活物抑制剂-1(PAI-1)基因多态性与严重烧伤患者脓毒症的相关性。方法选取142例烧伤面积>30%体表总面积的患者,其中烧伤后脓毒症患者82例(脓毒症组),烧伤后非脓毒症患者60例(对照组)。应用等位基因特异性扩增多聚酶联(AS-PCR)方法对两组行PAI-1基因多态性分析。酶联免疫吸附试验(ELISA)方法测定血浆PAI-l含量。结果①脓毒症组PAI-1 4G/4G基因型携带频率为43%,4G等位基因携带频率为62%;对照组PAI-1 4G/4G基因型携带频率为22%,4G等位基因携带频率为45%;两组比较差异有统计学意义(P<0.05)。②脓毒症组PAI-1抗原含量水平明显升高(P<0.05)。③PAI-1基因4G纯合子血浆PAI-1抗原水平高于5G纯合子血浆PAI-1抗原水平(P<0.05)。结论①PAI-1基因启动子区4G/5G多态性可能是烧伤脓毒症易感的危险因素,4G纯合子是其高危因素;②烧伤脓毒症患者血浆PAI-1抗原水平升高。Objective To study the correlation between burn sepsis and 4G/5G polymorphism within the promoter re-gion of plasminogen activator inhibitor-1 （ PAI-1） gene in severely burned patients.Methods A total of 142 patients with burn area larger than 30% of the total body surface area were collected.Peripheral blood samples were obtained from 82 patients with sepsis （the sepsis group） and 60 patients without sepsis （the control group）.4G/5G polymor-phism of PAI-1 gene was determined by using allele sepcial polymerase chain reaction （ AS-PCR） test.PAI-1 concen-trations were measured by enzyme-linked immunosorbent assay （ELISA）.Results The carrier frequency of 4G/4G genotype and the 4G allele frequency in the sepsis group were 43% and 62% respectively, which were significantly higher than that in the control group （P〈0.05）.Compared with the control group, PAI-1 concentrations in the sepsis group were significantly higher （P〈0.05）.Compared with the 5G/5G genotype, PAI-1 concentrations were signifi-cantly higher in the 4G/4G genotype （P〈0.05）.Conclusion The 4G/5G polymorphism within the promoter region of PAI-1 gene may be associated with the susceptibility of burn sepsis, and 4G/4G genotype may be the genetic risk factors of burn sepsis.The PAI-1 concentrations in serum are raised in patients with burn sepsis.

关 键 词：纤溶酶原激活物抑制剂-1 基因 多态性 烧伤 脓毒症 

分 类 号：R644[医药卫生—外科学]