离体和在体机械损伤致血管平滑肌增殖模型的建立  

Establishment of the Proliferation Model of Vascular Smooth Muscle Cells Induced by Mechanical Injury in Vitro and in Vivo

作　　者：高林江 刘幸平[2] 韩莎莎[2] 姜红岩[2] 李金玉[2] 黄展勤[2] 

机构地区：[1]汕头市中心血站,广东汕头515064 [2]汕头大学医学院药理学教研室,广东汕头515041

出　　处：《中国医药指南》2014年第22期1-2,共2页Guide of China Medicine

基　　金：国家自然科学基金资助项目(No.30901810);广东省自然科学基金资助项目(No.9151063201000072);汕头市重点科技计划项目(2008年度)

摘　　要：目的建立离体和在体机械损伤致血管平滑肌增殖模型。方法原代培养大鼠胸主动脉血管平滑肌细胞(VSMCs),体外建立机械划伤致VSMCs增殖模型,采用5-溴脱氧尿嘧啶(5-BrUd)掺入法,流式细胞术检测细胞增殖情况;整体动物上,采用新西兰兔左侧颈总动脉球囊损伤,术后7d取颈总动脉段,常规病理切片,HE染色,观测血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积。结果机械划痕损伤12 h后划伤处两侧的细胞开始有部分增殖和迁移,24 h后细胞增殖较为明显。流式细胞术检测机械损伤后细胞增殖增多(P<0.05)。整体动物上,与假手术组比较,模型组术后7 d血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积显著增加(P<0.01)。结论机械损伤可以导致离体和在体血管平滑肌细胞增殖。Objective To establish the proliferation model of vascular smooth muscle cells induced by mechanical injury in vitro and in vivo. Methods The primarily cultured rat thoracic aortic SMCs were used to establish mechanical injury models in vitro. The proliferation activity of VSMCs was analyzed by flow cytometry with 5-BrUd incorporation. The proliferation of vascular smooth muscle cells （VSMCs） in vivo was established by balloon injury on the rabbit carotid artery. After 7 days, experimental segments of arteries were harvested and subsequently progressed routine pathological sections, hematoxylin-eosin staining. Results The proliferation and migration of VSMCs were observed after 12h mechanical injury, especially after 24h. Compared with the sham group, the proliferative cells were increased by mechanical injury by the analysis of flow cytometry（P〈0.05）. Tthe intimal thickness, intimal area, intimal/medial thickness and intima-to-media area ratio of the Model group significantly increase comparing to the sham group （P〈0.01）. Conclusion The proliferation model of vascular smooth muscle cells induced by mechanical injury in vitro and in vivo were successfully established.

关 键 词：机械损伤 血管平滑肌细胞 增殖 离体 在体 

分 类 号：R654.2[医药卫生—外科学]