胡椒碱对乳腺癌阿霉素耐药细胞株MCF-7/ADM细胞的逆转作用  

Reversal Effect of Piperine on Adriamycin-Resistant MCF-7/ADM Cells of Breast Cancer

作　　者：赖艳[1] 温悦[1] 卢来春[1] 

机构地区：[1]中国人民解放军第三军医大学大坪医院野战外科研究所药剂科,重庆400042

出　　处：《中国药业》2014年第15期23-24,共2页China Pharmaceuticals

基　　金：重庆市卫生局中医药资助课题;项目编号:2011-2-115

摘　　要：目的探讨胡椒碱对人乳腺癌阿霉素(ADM)耐药细胞株MCF-7/ADM的逆转作用及其机制。方法采用CCK-8法观察胡椒碱联合阿霉素(ADM)对乳腺癌耐药株MCF-7/ADM细胞生长增殖的影响;应用免疫印迹技术(Western-blot)测定胡椒碱联合ADM对MCF-7/ADM细胞表面P-糖蛋白(P-gp)表达的影响。结果 60,80,100μmol/L胡椒碱试验组细胞耐药逆转倍数分别为1.62,2.08,3.78倍,差异有统计学意义(P<0.05)。胡椒碱能显著抑制MCF-7/ADM细胞膜P-gp蛋白的表达,其作用随浓度的增加而增强。结论胡椒碱与ADM共同作用于MCF-7/ADM细胞,可使细胞对ADM的敏感性增强。胡椒碱具有部分逆转MCF-7/ADM细胞耐药的作用,其逆转作用机制可能与通过下调P-gp的表达有关。Objective To explore the reversal effect and mechanism of piperine on the multidrug adriamycin（ADM） - resistance cell line MCF - 7 / ADM of human breast cancer. Methods The effect of piperine combined with ADM on growth and proliferation of drug - re-sistant MCF - 7 / ADM cells were observed by using the CCK - 8 assay. The expression levels of P - glycoprotein （P - gp） in MCF - 7 / ADM cells treated with piperine were detected by Western blot. Results The drug resistance reversal fold in the experimental groups with 60,80,100 μmol / L of piperine was 1. 62,2. 08,3. 78 times respectively. Piperine could significantly inhibit the expression of P - gp on the cytomembrane of MCF - 7 / ADM cells and enhanced the cellular sensitivity to ADM. Conclusion Piperine and adriamyci co - acting on MCF - 7 / ADM cells can increase the cellular sensitivity to ADM. Piperine can partially induce the reversal of drug resistance of MCF - 7 / ADM cells, its mechanism may be related with the down - regulation of the P - gp expression.

关 键 词：胡椒碱 多药耐药 P-糖蛋白 乳腺癌阿霉素耐药细胞株MCF-7 ADM细胞 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]