响应面法优化解淀粉芽孢杆菌C101发酵培养基  

Optimization of Fermentation Medium by Bacillus amyloliquefaciens Strain C101 Using Response Surface Methodology

作　　者：梁昌聪[1] 郭立佳[1] 刘磊[1] 张建华[1] 杨腊英[1] 王国芬[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,农业部热带作物有害生物综合治理重点实验室,海南省热带农业有害生物检测与控制重点实验室,海口571101

出　　处：《生物技术通报》2014年第8期169-174,共6页Biotechnology Bulletin

基　　金：国家公益性行业(农业)科研项目(200903049)

摘　　要：采用响应面法对解淀粉芽孢杆菌C101菌株产芽孢发酵培养基进行了优化。利用Plackett-Burman试验设计筛选出影响产孢的3个主要因素:MnSO4、KH2PO4和(NH4)2SO4。在此基础上运用最陡爬坡路径法逼近最大响应值区域,最后利用响应面分析法确定主要因子之间的交互作用及最佳条件。结果表明,蔗糖20 g/L,尿素4.0 g/L,豆粕4.0 g/L,KNO3 2.0 g/L,Na2HPO4 2.4 g/L,KH2PO4 0.52 g/L,(NH4)2SO4 0.55 g/L,NaCl 1.0 g/L,MgSO4·7H2O 0.50 g/L,FeSO4 0.005 0 g/L,MnSO4 0.005 4 g/L,C101最大理论芽孢含量为14.67×108个/mL。经3次平行试验验证,实际平均芽孢含量与预测芽孢含量相近,比之前的芽孢含量提高了188%。The fermentation medium components for spore content by Bacillus amyloliquefaciens strain C101 was optimized by response surface methodology（RSM）. Firstly, three of the most significant influence factors were screened by the method of Plackett-Burman design as MnSO4, KH2PO4 and（NH4）2SO4. The path of steepest ascent was applied to approach the optimal region of the three significant factors. Lastly, the optimal concentration and correlations among three factors were identified by RSM. The optimal fermentation conditions were determined as followed：sucrose 20 g/L, urea 4.0 g/L, soybean meal 4.0 g/L, KNO3 2.0 g/L, Na2HPO4 2.4 g/L, KH2PO4 0.52 g/L,（NH4）2SO4 0.55 g/L, NaCl 1.0 g/L, MgSO4·7H2O 0.50 g/L, FeSO4 0.005 0 g/L, MnSO4 0.005 4 g/L. Under these conditions, the maximum theoretic spore number was 14.67＆#215;108/mL. After three parallel verifications, the maximum theoretic value was consistent with mean value of verification test and the spore number was increased by 188%compare to that before optimization.

关 键 词：响应面法 解淀粉芽孢杆菌 芽孢 优化 

分 类 号：Q93-335[生物学—微生物学]