蓝莓最佳继代培养基的筛选试验  

Screening Test on the Best Subculture Medium for Blueberry

作　　者：梁文卫[1] 

机构地区：[1]黑龙江省农业科学院浆果研究所,黑龙江绥棱152204

出　　处：《黑龙江农业科学》2014年第8期32-34,共3页Heilongjiang Agricultural Sciences

摘　　要：为解决蓝莓组培过程中分化率低的问题,从而获得蓝莓的最佳继代培养基,以矮丛蓝莓品种美登的无根试管苗为试材,采用正交试验的方法,设计四因素混合水平的继代培养基进行研究。结果表明:最适宜蓝莓的继代培养基是改良的WPM+ZT 0.5mg·L-1+IBA 0.1mg·L-1+蔗糖25g·L-1,pH5.0。In order to solve the prombie of low differentiation rate during tissue culture of blueberry,and obtain the best subculture medium for blueberry, taking the rootless piantlets seedling of rosette blueberry variety （Blomidon） as materials, four factors mixed levels of subculture medium was studied by orthogonal experiment. The results showed that the optimum subculture medium for blueberry was improved with WPM＋ZT 0.5 mg·L-1 ＋IBA 0.1 mg·L-1 d-sucrose 25 g·L-1 ,and the pH was 5.0.

关 键 词：蓝莓 正交试验 继代培养基 

分 类 号：S663.9[农业科学—果树学]